Document Detail


Expression of antifreeze proteins in transgenic plants.
MedLine Citation:
PMID:  1932678     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The quality of frozen fruits and vegetables can be compromised by the damaging effects of ice crystal growth within the frozen tissue. Antifreeze proteins in the blood of some polar fishes have been shown to inhibit ice recrystallization at low concentrations. In order to determine whether expression of genes of this type confers improved freezing properties to plant tissue, we have produced transgenic tobacco and tomato plants which express genes encoding antifreeze proteins. The afa3 antifreeze gene was expressed at high steady-state mRNA levels in leaves from transformed plants, but we did not detect inhibition of ice recrystallization in tissue extracts. However, both mRNA and fusion proteins were detectable in transgenic tomato tissue containing a chimeric gene encoding a fusion protein truncated staphylococcal protein A and antifreeze protein. Furthermore, ice recrystallization inhibition was detected in this transgenic tissue.
Authors:
R Hightower; C Baden; E Penzes; P Lund; P Dunsmuir
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant molecular biology     Volume:  17     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1991-11-25     Completed Date:  1991-11-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1013-21     Citation Subset:  IM    
Affiliation:
DNA Plant Technology Corporation, Oakland, CA 94608.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antifreeze Proteins
Chimera
Cloning, Molecular
Escherichia coli / genetics
Fishes
Freezing
Glycoproteins / biosynthesis,  genetics*,  isolation & purification
Ice
Molecular Sequence Data
Molecular Weight
Plants, Genetically Modified*
Plants, Toxic*
Recombinant Proteins / biosynthesis,  isolation & purification
Restriction Mapping
Tobacco / genetics*
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Antifreeze Proteins; 0/Glycoproteins; 0/Ice; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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