| Expression of antifreeze proteins in transgenic plants. | |
MedLine Citation:
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PMID: 1932678 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The quality of frozen fruits and vegetables can be compromised by the damaging effects of ice crystal growth within the frozen tissue. Antifreeze proteins in the blood of some polar fishes have been shown to inhibit ice recrystallization at low concentrations. In order to determine whether expression of genes of this type confers improved freezing properties to plant tissue, we have produced transgenic tobacco and tomato plants which express genes encoding antifreeze proteins. The afa3 antifreeze gene was expressed at high steady-state mRNA levels in leaves from transformed plants, but we did not detect inhibition of ice recrystallization in tissue extracts. However, both mRNA and fusion proteins were detectable in transgenic tomato tissue containing a chimeric gene encoding a fusion protein truncated staphylococcal protein A and antifreeze protein. Furthermore, ice recrystallization inhibition was detected in this transgenic tissue. |
Authors:
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R Hightower; C Baden; E Penzes; P Lund; P Dunsmuir |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant molecular biology Volume: 17 ISSN: 0167-4412 ISO Abbreviation: Plant Mol. Biol. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1991-11-25 Completed Date: 1991-11-25 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9106343 Medline TA: Plant Mol Biol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1013-21 Citation Subset: IM |
Affiliation:
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DNA Plant Technology Corporation, Oakland, CA 94608. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antifreeze Proteins Chimera Cloning, Molecular Escherichia coli / genetics Fishes Freezing Glycoproteins / biosynthesis, genetics*, isolation & purification Ice Molecular Sequence Data Molecular Weight Plants, Genetically Modified* Plants, Toxic* Recombinant Proteins / biosynthesis, isolation & purification Restriction Mapping Tobacco / genetics* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Antifreeze Proteins; 0/Glycoproteins; 0/Ice; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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