| Expression and analysis of two novel rat organic cation transporter homologs, SLC22A17 and SLC22A23. | |
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MedLine Citation:
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PMID: 21359964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The organic cation transporter (OCT, SLC22) family is a family of polyspecific transmembrane proteins that are responsible for the uptake or excretion of many cationic drugs, toxins, and endogenous metabolites in a variety of tissues. Many of the OCTs have been previously characterized, but there are a number of orphan genes whose functions remain unknown. In this study, two novel rat SLC22 genes, SLC22A17 (BOCT1) and SLC22A23 (BOCT2), were cloned and characterized. Northern blot analysis showed that BOCT1 and BOCT2 mRNA was expressed in a wide variety of tissues. BOCT1 was strongly expressed in brain, primary neurons and brain endothelial cells, with highest expression in choroid plexus. BOCT2 was also abundantly expressed in brain, as well as in liver. To characterize the products of these genes, BOCT1 cDNA was isolated from a rat blood-brain barrier cDNA library, and BOCT2 cDNA was isolated from rat brain capillary and from cultured neurons using PCR techniques. Plasmids expressing BOCT1 and BOCT2 were transfected into HEK-293 cells, as were control cDNAs for OCT1 and OCTN2. Recombinant cell surface protein was verified by western blot and fluorescence microscopy. Transport activity of BOCT1 and BOCT2 was evaluated using radioisotope uptake assays. The OCT1- and OCTN2-expressing cells transported the canonical substrates, 1-methyl-4-phenyl-pyridinium (MPP(+)) and carnitine, respectively. However, BOCT1 and BOCT2-expressing cells did not show transport activity for these substrates or a number of other SLC22 substrates. These novel family members have a nonconserved amino terminus, relative to other OCTs, that may preclude typical SLC22 transport function. |
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Authors:
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Katie M Bennett; Jun Liu; Courtney Hoelting; James Stoll |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-27 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 352 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-18 Completed Date: 2011-09-30 Revised Date: 2012-09-18 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 143-54 Citation Subset: IM |
Affiliation:
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Department of Biomedical Sciences, Texas Tech University Health Sciences Center, School of Pharmacy, Amarillo, TX 79106, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Blotting, Western DNA Primers DNA, Complementary Microscopy, Fluorescence Molecular Sequence Data Organic Cation Transport Proteins / chemistry, genetics* RNA, Messenger / genetics Rats Sequence Homology, Amino Acid |
| Grant Support | |
ID/Acronym/Agency:
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R15 GM070578/GM/NIGMS NIH HHS; R15 GM070578-01/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Complementary; 0/Organic Cation Transport Proteins; 0/RNA, Messenger |
| Comments/Corrections | |
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