Document Detail

Expression of allene oxide cyclase and accumulation of jasmonates during organogenic nodule formation from hop (Humulus lupulus var. Nugget) internodes.
MedLine Citation:
PMID:  16100229     Owner:  NLM     Status:  MEDLINE    
A crucial step in the biosynthesis of jasmonic acid (JA) is the formation of its stereoisomeric precursor, cis-(+)-12-oxophytodienoic acid (OPDA), which is catalyzed by allene oxide cyclase (AOC, EC A cDNA of AOC was isolated from Humulus lupulus var. Nugget. The ORF of 765 bp encodes a 255 amino acid protein, which carries a putative chloroplast targeting sequence. The recombinant protein without its putative chloroplast target sequence showed significant AOC activity. Previously we demonstrated that wounding induces organogenic nodule formation in hop. Here we show that the AOC transcript level increases in response to wounding of internodes, peaking between 2 and 4 h after wounding. In addition, Western blot analysis showed elevated levels of AOC peaking 24 h after internode inoculation. The AOC increase was accompanied by increased JA levels 24 h after wounding, whereas OPDA had already reached its highest level after 12 h. AOC is mostly present in the vascular bundles of inoculated internodes. During prenodule and nodule formation, AOC levels were still high. JA and OPDA levels decreased down to 10 and 118 pmol (g FW)(-1), respectively, during nodule formation, but increased during plantlet regeneration. Double immunolocalization analysis of AOC and Rubisco in connection with lugol staining showed that AOC is present in amyloplasts of prenodular cells and in the chloroplasts of vacuolated nodular cells, whereas meristematic cells accumulated little AOC. These data suggest a role of AOC and jasmonates in organogenic nodule formation and plantlet regeneration from these nodules.
Ana M Fortes; Otto Miersch; Peter R Lange; Rui Malhó; Pilar S Testillano; Maria Del Carmen Risueño; Claus Wasternack; Maria S Pais
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-08-12
Journal Detail:
Title:  Plant & cell physiology     Volume:  46     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-11-09     Completed Date:  2006-01-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1713-23     Citation Subset:  IM    
Laboratory of Molecular Biology and Plant Biotechnology, Ed. ICAT, FCUL, Campo Grande, Lisboa, Portugal.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY644677;  AY745883
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cyclopentanes / metabolism*
DNA Primers
Escherichia coli / genetics
Humulus / metabolism*
Intramolecular Oxidoreductases / chemistry,  genetics,  metabolism*
Molecular Sequence Data
Plant Roots / metabolism*
RNA, Messenger / genetics
Sequence Homology, Amino Acid
Reg. No./Substance:
0/Cyclopentanes; 0/DNA Primers; 0/Oxylipins; 0/RNA, Messenger; 6894-38-8/jasmonic acid; EC 5.3.-/Intramolecular Oxidoreductases; EC isomerase

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