Document Detail


Expression of adiponectin and its receptors (AdipoR1 and AdipoR2) in chicken ovary: potential role in ovarian steroidogenesis.
MedLine Citation:
PMID:  17010558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adiponectin and its receptors (AdipoR1 and AdipoR2) mRNAs are expressed in various chicken tissues including ovary. However, the cellular expression and the role of adiponectin system have never been investigated in chicken ovary. Here, we have shown that the level of adiponectin mRNA is about 10- to 30-fold higher (p<0.001) in theca cells than in granulosa cells from each hierarchical yellow follicle studied (F4-F1). In contrast, the level of AdipoR1 mRNA expression was about two-fold lower in theca cells than in granulosa cells (p<0.05) whereas those of AdipoR2 was similar in both ovarian cells. Whereas expression of adiponectin mRNA increased with follicular differentiation in theca cells, it decreased in granulosa cells. In contrast, mRNA expression of AdipoR1 and AdipoR2 in both theca and granulosa cells remained stable during yellow follicle development. To determine whether adiponectin is involved in the ovarian steroidogenesis, LH (100 ng/ml)-, FSH (100 ng/ml)- and IGF-1 (100 ng/ml)-induced progesterone production was measured in absence or presence of human recombinant adiponectin (10 microg/ml) for 36 h in cultured granulosa cells from F1, F2 and mixed F3 and F4 follicles. In absence of LH, FSH and IGF-1, adiponectin treatment had no effects on progesterone production whatever vitollegenic follicle studied. However, it increased by about two-fold IGF-1-induced progesterone secretion in F2 and F3/4 follicles whereas it halved progesterone production in response to gonadotropins (LH and FSH) in F3/4 follicles. Thus, in chicken, adiponectin, mainly expressed in theca cells, could exert paracrine or autocrine effect on the ovarian steroidogenesis.
Authors:
Christine Chabrolle; Lucie Tosca; Sabine Crochet; Sophie Tesseraud; Joëlle Dupont
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Publication Detail:
Type:  Journal Article     Date:  2006-09-18
Journal Detail:
Title:  Domestic animal endocrinology     Volume:  33     ISSN:  0739-7240     ISO Abbreviation:  Domest. Anim. Endocrinol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-23     Completed Date:  2007-12-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8505191     Medline TA:  Domest Anim Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  480-7     Citation Subset:  IM    
Affiliation:
Unité de Physiologie de Reproduction et des Comportements, Institut National de Recherche Agronomique, 37380 Nouzilly, France.
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MeSH Terms
Descriptor/Qualifier:
AMP-Activated Protein Kinases
Acetyl-CoA Carboxylase / metabolism
Adiponectin / genetics*,  pharmacology,  physiology
Animals
Chickens / metabolism*
Female
Follicle Stimulating Hormone / pharmacology
Gene Expression*
Humans
Insulin-Like Growth Factor I / pharmacology
Luteinizing Hormone / pharmacology
Multienzyme Complexes / metabolism
Ovary / metabolism*
Progesterone / biosynthesis*
Protein-Serine-Threonine Kinases / metabolism
Receptors, Adiponectin / genetics*,  physiology
Recombinant Proteins / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Adiponectin; 0/Multienzyme Complexes; 0/Receptors, Adiponectin; 0/Recombinant Proteins; 57-83-0/Progesterone; 67763-96-6/Insulin-Like Growth Factor I; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone; EC 2.7.11.1/AMP-Activated Protein Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 6.4.1.2/Acetyl-CoA Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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