Document Detail


Expression of adhesion and activation molecules in human buccal epithelial cell lines and normal human buccal epithelium in situ.
MedLine Citation:
PMID:  11168856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An in vitro culture system may be used to analyse interactions between T cells and epithelium. We have analysed two human buccal squamous epithelial cell lines: SqCC/Y1, derived from a buccal carcinoma, and SVpgC2a, an SV-transformed healthy buccal squamous epithelium. Under serum-free culture conditions, the cell lines resemble normal buccal squamous epithelium in situ in their expression of MHC class I, CD29 (beta1 integrin), CD40, CD44, CD54 (ICAM-1), CD58 (LFA-3), CD95 (Fas), and E-cadherin. Furthermore, when the SVpgC2a cell line was treated with T-cell derived cytokines, upregulation of CD40 expression was observed when the cells were treated with IL-4, whereas IFN-gamma caused upregulation in expression of CD40, CD54 and MHC class II. These buccal squamous epithelial cell lines, therefore, provide a good tool for analysing interactions between epithelium and T cells in vitro.
Authors:
I Farmer; J Freysdottir; A M Dalghous; F Fortune
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  30     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-06     Completed Date:  2001-04-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  113-20     Citation Subset:  D; IM    
Affiliation:
Department of Oral Medicine, Leeds Dental Institute, UK.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD29 / analysis
Antigens, CD40 / analysis
Antigens, CD44 / analysis
Antigens, CD58 / analysis
Antigens, CD95 / analysis
Cadherins / analysis
Carcinoma, Squamous Cell / pathology
Cell Adhesion Molecules / analysis*,  genetics
Cell Line
Cell Line, Transformed
Cell Transformation, Viral
Culture Media, Serum-Free
Cytokines / pharmacology
Epithelial Cells / cytology,  pathology
Flow Cytometry
Gene Expression Regulation
Histocompatibility Antigens Class I / analysis
Humans
Intercellular Adhesion Molecule-1 / analysis
Interferon-gamma / pharmacology
Interleukin-4 / pharmacology
Mouth Mucosa / cytology*,  pathology
Mouth Neoplasms / pathology
Simian virus 40
Tumor Cells, Cultured
Up-Regulation
Chemical
Reg. No./Substance:
0/Antigens, CD29; 0/Antigens, CD40; 0/Antigens, CD44; 0/Antigens, CD58; 0/Antigens, CD95; 0/Cadherins; 0/Cell Adhesion Molecules; 0/Culture Media, Serum-Free; 0/Cytokines; 0/Histocompatibility Antigens Class I; 126547-89-5/Intercellular Adhesion Molecule-1; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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