| Expression and adaptive regulation of amino acid transport system A in a placental cell line under amino acid restriction. | |
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MedLine Citation:
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PMID: 16672359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trans-placental transport of amino acids is vital for the developing fetus. Using the BeWo cell line as a placental model, we investigated the effect of restricting amino acid availability on amino acid transport system type A. BeWo cells were cultured either in amino acid-depleted (without non-essential amino acids) or control media for 1, 3, 5 or 6 h. System A function was analysed using alpha(methyl-amino)isobutyric acid (MeAIB) transcellular transport studies. Transporter (sodium coupled neutral amino acid transporter (SNAT1/2)) expression was analysed at mRNA and protein level by Northern and Western blotting respectively. Localisation was carried out using immunocytochemistry. MeAIB transcellular transport was significantly (P < 0.05) increased by incubation of the cells in amino acid-depleted medium for 1 h, and longer incubation times caused further increases in the rate of transfer. However, the initial response was not accompanied by an increase in SNAT2 mRNA; this occurred only after 3 h and further increased for the rest of the 6-h incubation. Similarly, it took several hours for a significant increase in SNAT2 protein expression. In contrast, relocalisation of existing SNAT2 transporters occurred within 30 min of amino acid restriction and continued throughout the 6-h incubation. When the cells were incubated in medium with even lower amino acid levels (without non-essential plus 0.5 x essential amino acids), SNAT2 mRNA levels showed further significant (P < 0.0001) up-regulation. However, incubation of cells in depleted medium for 6 h caused a significant (P = 0.014) decrease in the expression of SNAT1 mRNA. System L type amino acid transporter 2 (LAT2) expression was not changed by amino acid restriction, indicating that the responses seen in the system A transporters were not a general cell response. These data have shown that placental cells adapt in vitro to nutritional stress and have identified the physiological, biochemical and genomic mechanisms involved. |
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Authors:
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H N Jones; C J Ashworth; K R Page; H J McArdle |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproduction (Cambridge, England) Volume: 131 ISSN: 1470-1626 ISO Abbreviation: Reproduction Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-05-04 Completed Date: 2006-11-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100966036 Medline TA: Reproduction Country: England |
Other Details:
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Languages: eng Pagination: 951-60 Citation Subset: IM |
Affiliation:
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Maternal-Fetal Physiology, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Transport System A
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analysis,
genetics*,
metabolism Amino Acid Transport System y+ / genetics, metabolism Amino Acids / deficiency*, metabolism Analysis of Variance Antigens, CD98 Light Chains / genetics, metabolism Biological Transport Blotting, Northern / methods Blotting, Western / methods Cell Line, Tumor Choriocarcinoma Culture Media Electric Impedance Epithelium / metabolism Female Gene Expression Humans Immunohistochemistry / methods Placenta / metabolism* RNA, Messenger / analysis* Trophoblasts / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acid Transport System A; 0/Amino Acid Transport System y+; 0/Amino Acids; 0/Antigens, CD98 Light Chains; 0/Culture Media; 0/RNA, Messenger; 0/SLC38A2 protein, human; 0/SLC7A8 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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