| Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis. | |
| | |
MedLine Citation:
|
PMID: 20180636 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cysteine cathepsins and matrix metalloproteases are considered to play important roles in the development of arthritic diseases. Their accumulation in synovial fluid of primarily rheumatoid arthritis patients is also well documented. However, a detailed comparison between the protease levels and activities between rheumatoid arthritis samples and osteoarthritis samples has never been made. Here, we report that both cysteine cathepsins B and S and matrix metalloproteases-1, -3 and -13 are detected in patient synovial fluid samples with significantly higher levels detected in rheumatoid arthritis patients. Among the proteases, cathepsin S was found to be significantly elevated, consistent with its critical role in the immune response. These results suggest that cysteine cathepsins have a major role in inflammation at least in rheumatoid arthritis. In addition to proteases, interleukin-6 was detected at significant levels in most samples, suggesting that proinflammatory cytokines might be in-volved in the stimulation of expression of these proteases during inflammation. |
| | |
Authors:
|
Urska Pozgan; Dejan Caglic; Blaz Rozman; Hideaki Nagase; Vito Turk; Boris Turk |
Related Documents
:
|
17122006 - Distribution of mycoplasma pneumoniae and mycoplasma salivarium in the synovial fluid o... 20144096 - Thermography in juvenile rheumatoid arthritis. 9529556 - Pruritus in hiv-1 disease: therapy with drugs which may modulate the pattern of immune ... 8948296 - Remission in a prospective study of patients with rheumatoid arthritis. american rheuma... 12449176 - Steroid treatment based on the findings of transbronchial biopsy in idiopathic intersti... 3451216 - Should patients with haemoptysis and a normal chest x-ray be bronchoscoped? |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biological chemistry Volume: 391 ISSN: 1437-4315 ISO Abbreviation: Biol. Chem. Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-05-20 Completed Date: 2010-08-20 Revised Date: 2011-12-01 |
Medline Journal Info:
|
Nlm Unique ID: 9700112 Medline TA: Biol Chem Country: Germany |
Other Details:
|
Languages: eng Pagination: 571-9 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Arthritis, Rheumatoid / enzymology* Cathepsin B / isolation & purification*, metabolism Cathepsins / isolation & purification*, metabolism Cystatin C / metabolism Female Humans Interleukin-1beta / metabolism Interleukin-6 / metabolism Male Matrix Metalloproteinase 1 / metabolism Matrix Metalloproteinase 13 / metabolism Matrix Metalloproteinase 3 / metabolism Matrix Metalloproteinases / isolation & purification, metabolism* Middle Aged Osteoarthritis / enzymology* Synovial Fluid / enzymology* Tumor Necrosis Factor-alpha / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
//Arthritis Research UK |
| Chemical | |
Reg. No./Substance:
|
0/Cystatin C; 0/Interleukin-1beta; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha; EC 3.4.-/Cathepsins; EC 3.4.22.1/CTSB protein, human; EC 3.4.22.1/Cathepsin B; EC 3.4.22.27/cathepsin S; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.17/Matrix Metalloproteinase 3; EC 3.4.24.7/Matrix Metalloproteinase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Klk8, a multifunctional protease in the brain and skin: analysis of knockout mice.
Next Document: Kallikrein-related peptidases: bridges between immune functions and extracellular matrix degradation...