Document Detail


Expression and activity of mTOR and its substrates in different cell cycle phases and in oral squamous cell carcinomas of different malignant grade.
MedLine Citation:
PMID:  16927414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to study the relationship between mTOR (mammalian target of rapamycin) and tumorigenesis, we investigated the expression and activity of mTOR, and its substrates, alpha1, alpha2, beta1 and beta2 isoforms of p70S6 kinase (p70S6K) and eukaryotic initiation factor 4E binding protein-1 (4EBP-1) in oral squamous carcinoma and Hela cells using RT-PCR, immunohistochemistry, statistical analysis and Western blotting. The result of Western blots showed that in poorly differentiated oral squamous carcinoma, the expression level of mTOR and p70S6k increased in M phase, while that of 4EBP-1 decreased. The results of RT-PCR and immunohistochemistry assay are the same as that of Western blot. In Hela cells, the RT-PCR results showed that the level of mTOR mRNA did not change during the cell cycle. In M phase, the expression of alpha1, alpha2, beta1 and beta2 isoforms of p70S6K increased noticeably, while the expression of 4EBP-1 decreased. The immunoblot results in Hela cells were consistent with the RT-PCR results. Furthermore, the activity assays in Hela cells suggested that,in phase G2 and M, the activity of mTOR was maintained at a higher level than in any other phase, while 4EBP-1 decreased in phase M. These results may help in further investigations of the important role of mTOR in cell cycle and tumorigenesis.
Authors:
Yi Liu; Sujuta Hidayat; Wen-hui Su; Xin Deng; Da-hai Yu; Bing-zhi Yu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  25     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:    2007 Jan-Feb
Date Detail:
Created Date:  2006-12-25     Completed Date:  2007-02-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  45-53     Citation Subset:  IM    
Copyright Information:
2006 John Wiley & Sons, Ltd.
Affiliation:
Orthodontic Department of Dentistry College, China Medical University, Shenyang 110001, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / genetics,  metabolism*
Carcinoma, Squamous Cell / genetics,  metabolism*,  pathology*
Cell Cycle
Gene Expression
Gene Expression Regulation, Neoplastic
Hela Cells
Humans
Immunohistochemistry
Isoenzymes / genetics,  metabolism
Mouth Neoplasms / genetics,  metabolism*,  pathology*
Neoplasm Staging
Phosphoproteins / genetics,  metabolism*
Protein Kinases / genetics,  metabolism*
RNA, Messenger / genetics
Ribosomal Protein S6 Kinases, 70-kDa / genetics,  metabolism*
Substrate Specificity
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/EIF4EBP1 protein, human; 0/Isoenzymes; 0/Phosphoproteins; 0/RNA, Messenger; EC 2.7.-/Protein Kinases; EC 2.7.1.-/mTOR protein; EC 2.7.11.1/Ribosomal Protein S6 Kinases, 70-kDa

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