| Expression and activity of mTOR and its substrates in different cell cycle phases and in oral squamous cell carcinomas of different malignant grade. | |
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MedLine Citation:
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PMID: 16927414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to study the relationship between mTOR (mammalian target of rapamycin) and tumorigenesis, we investigated the expression and activity of mTOR, and its substrates, alpha1, alpha2, beta1 and beta2 isoforms of p70S6 kinase (p70S6K) and eukaryotic initiation factor 4E binding protein-1 (4EBP-1) in oral squamous carcinoma and Hela cells using RT-PCR, immunohistochemistry, statistical analysis and Western blotting. The result of Western blots showed that in poorly differentiated oral squamous carcinoma, the expression level of mTOR and p70S6k increased in M phase, while that of 4EBP-1 decreased. The results of RT-PCR and immunohistochemistry assay are the same as that of Western blot. In Hela cells, the RT-PCR results showed that the level of mTOR mRNA did not change during the cell cycle. In M phase, the expression of alpha1, alpha2, beta1 and beta2 isoforms of p70S6K increased noticeably, while the expression of 4EBP-1 decreased. The immunoblot results in Hela cells were consistent with the RT-PCR results. Furthermore, the activity assays in Hela cells suggested that,in phase G2 and M, the activity of mTOR was maintained at a higher level than in any other phase, while 4EBP-1 decreased in phase M. These results may help in further investigations of the important role of mTOR in cell cycle and tumorigenesis. |
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Authors:
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Yi Liu; Sujuta Hidayat; Wen-hui Su; Xin Deng; Da-hai Yu; Bing-zhi Yu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell biochemistry and function Volume: 25 ISSN: 0263-6484 ISO Abbreviation: Cell Biochem. Funct. Publication Date: 2007 Jan-Feb |
Date Detail:
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Created Date: 2006-12-25 Completed Date: 2007-02-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: England |
Other Details:
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Languages: eng Pagination: 45-53 Citation Subset: IM |
Copyright Information:
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2006 John Wiley & Sons, Ltd. |
Affiliation:
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Orthodontic Department of Dentistry College, China Medical University, Shenyang 110001, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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genetics,
metabolism* Carcinoma, Squamous Cell / genetics, metabolism*, pathology* Cell Cycle Gene Expression Gene Expression Regulation, Neoplastic Hela Cells Humans Immunohistochemistry Isoenzymes / genetics, metabolism Mouth Neoplasms / genetics, metabolism*, pathology* Neoplasm Staging Phosphoproteins / genetics, metabolism* Protein Kinases / genetics, metabolism* RNA, Messenger / genetics Ribosomal Protein S6 Kinases, 70-kDa / genetics, metabolism* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/EIF4EBP1 protein, human; 0/Isoenzymes; 0/Phosphoproteins; 0/RNA, Messenger; EC 2.7.-/Protein Kinases; EC 2.7.1.-/mTOR protein; EC 2.7.11.1/Ribosomal Protein S6 Kinases, 70-kDa |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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