Document Detail

Expression of attaching/effacing activity by enteropathogenic Escherichia coli depends on growth phase, temperature, and protein synthesis upon contact with epithelial cells.
MedLine Citation:
PMID:  8641808     Owner:  NLM     Status:  MEDLINE    
Enteropathogenic Escherichia coli (EPEC) induces tyrosine phosphorylation of a 90-kDa protein (Hp90) in infected epithelial cells. This in turn facilitates intimate binding of EPEC via the outer membrane protein intimin, effacement of host cell microvilli, cytoskeletal rearrangement, and bacterial uptake. This phenotype has been commonly referred to as attaching/effacing (A/E). The ability of EPEC to induce A/E lesions was dependent on bacterial growth phase and temperature. Early-logarithmic-phase EPEC grown at 37 degrees C elicits strong A/E activity within minutes after infection of HeLa epithelial cells. EPEC de novo protein syntheses during the first minutes of interaction with the host cell was required to elicit A/E lesions. However, once formed, bacterial viability was not needed to maintain A/E lesions. The type of growth media and partial O2 pressure level do not seem to affect the ability of EPEC to cause A/E lesions. These results indicates that the A/E activity of EPEC is tightly regulated by environmental and host factors.
I Rosenshine; S Ruschkowski; B B Finlay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  64     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-07-16     Completed Date:  1996-07-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  966-73     Citation Subset:  IM    
Department of Biotechnology and Molecular Genetics, Faculty of Medicine, The Hebrew University, Jerusalem, Israel.
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MeSH Terms
Adhesins, Bacterial*
Bacterial Outer Membrane Proteins / physiology*
Bacterial Proteins / biosynthesis*
Carrier Proteins*
Escherichia coli / growth & development,  metabolism,  pathogenicity*
Escherichia coli Proteins*
Hela Cells
Tyrosine / metabolism
Reg. No./Substance:
0/Adhesins, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Escherichia coli Proteins; 147094-99-3/eaeA protein, E coli; 55520-40-6/Tyrosine

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