Document Detail

Expression and activation of Akt/protein kinase B in sexually immature and mature rat uterus.
MedLine Citation:
PMID:  15336705     Owner:  NLM     Status:  MEDLINE    
This study investigated the expression and activation of Akt/PKB in developing and adult rat uterus. Expression of Akt was observed in uteri from adult ovariectomized and 7-35-day-old rats and no changes were observed in response to in vivo estradiol treatment (1-100 microg/100g b.w.). To examine the mechanisms of PKB/Akt activation, phosphorylation at Thr(308) and Ser(473) regulatory sites were studied in uteri. Akt was constitutively phosphorylated on Ser(473) residue in the untreated, control uteri, while phosphorylation of Thr(308) was observed only after estradiol 17beta (E2) treatment. The effects of E2 treatment were age dependent, no response was induced in 11-day-old uteri, while in 28 days and older rats the activation of Akt at both regulatory sites, Ser(473) and Thr(308), increased, the first response was detected 2h after treatment, reaching the highest rate at 6h. The rate of phosphorylation was stronger at Ser(473) residue. The results suggest that the regulation of Akt activation at two regulatory sites in rat uteri are different, phosphorylation of Thr(308) seems to be entirely estrogen dependent, while the phosphorylation of Ser(473) is regulated by other factors as well as estrogen.
Ferenc Lengyel; Zsuzsanna Vértes; Kálmán A Kovács; József L Környei; Balázs Sumegi; Marietta Vértes
Related Documents :
16382195 - Effects of some new antidepressant drugs on the glucocorticoid receptor-mediated gene t...
12006525 - Dysregulation of pten and protein kinase b is associated with glioma histology and pati...
11097835 - Activation of akt is induced by heat shock and involved in suppression of heat-shock-in...
16085355 - Lack of correlation between leptin receptor expression and pi3-k/akt signaling pathway ...
10575015 - Activation of src family kinase yes induced by shiga toxin binding to globotriaosyl cer...
2153665 - Specificities of autoinhibitory domain peptides for four protein kinases. implications ...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of steroid biochemistry and molecular biology     Volume:  91     ISSN:  0960-0760     ISO Abbreviation:  J. Steroid Biochem. Mol. Biol.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-31     Completed Date:  2004-11-02     Revised Date:  2012-06-22    
Medline Journal Info:
Nlm Unique ID:  9015483     Medline TA:  J Steroid Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  285-8     Citation Subset:  IM    
Institute of Physiology, Pécs University Medical School, Szigeti út 12, Pécs H7624, Hungary.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Age Distribution
Enzyme Activation / drug effects*
Estradiol / pharmacology
Gene Expression Regulation, Developmental*
Phosphorylation / drug effects
Protein-Serine-Threonine Kinases / metabolism*
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-akt
Rats, Wistar
Serine / chemistry
Threonine / chemistry
Uterus / enzymology*,  growth & development*
Reg. No./Substance:
0/Proto-Oncogene Proteins; 50-28-2/Estradiol; 56-45-1/Serine; 72-19-5/Threonine; EC protein, rat; EC Kinases; EC Proteins c-akt

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Specific interactions of steroids, arylhydrocarbons and flavonoids with progesterone receptors from ...
Next Document:  Epidemiological evidence on reproductive effects of persistent organochlorines in humans.