Document Detail

Expression of VEGF, VEGF-C and VEGFR-2 in in situ and invasive SCC of cervix.
MedLine Citation:
PMID:  20036889     Owner:  NLM     Status:  MEDLINE    
Cervical squamous cell carcinoma (SCC) arises from the metaplastic epithelium and develops slowly through dysplastic changes (i.e., cervical intraepithelial neoplasia--CIN) to carcinoma in situ and invasive cancer. There is little data concerning the quantitation of vascular endothelial growth factor (VEGF) and its correlation to the clinical or pathologic characteristics of SCC. This study assessed the expression of VEGF, VEGF-C and their receptor VEGFR-2 in 35 samples of normal cervical tissue, 35--CIN1, 35--CIN2 (25 non-pregnant, 15 pregnant women), 35--CIN3 and 30- SCC. VEGF, VEGF-C and VEGFR-2 were analyzed using RT-PCR, RQ-PCR, immunohistochemical staining and Western blot. VEGF, VEGF-C and VEGFR-2 were not detected in normal cervical epithelium. In CIN and SCC, both forms of VEGF and its receptor were identified, indicating a correlation between the increasing expression and staging of carcinoma. Results show the important role of VEGF in cervical progression and that the switch to the lymphangiogenesis phenotype occurs prior to the stage of invasion likely at CIN2/3.
Robert Jach; Joanna Dulinska-Litewka; Piotr Laidler; Andrzej Szczudrawa; Andrzej Kopera; Lukasz Szczudlik; Michal Pawlik; Krzysztof Zajac; Monika Mak; Antoni Basta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-01
Journal Detail:
Title:  Frontiers in bioscience (Elite edition)     Volume:  2     ISSN:  1945-0508     ISO Abbreviation:  Front Biosci (Elite Ed)     Publication Date:  2010  
Date Detail:
Created Date:  2009-12-28     Completed Date:  2010-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101485240     Medline TA:  Front Biosci (Elite Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  411-23     Citation Subset:  IM    
Department of Gynecology, Obstetrics and Oncology, Jagiellonian University, Medical College, Krakow, Poland.
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MeSH Terms
Blotting, Western
Carcinoma, Squamous Cell / metabolism,  physiopathology*
Neoplasm Invasiveness / physiopathology*
Reverse Transcriptase Polymerase Chain Reaction
Uterine Cervical Neoplasms / metabolism,  physiopathology*
Vascular Endothelial Growth Factor A / metabolism*
Vascular Endothelial Growth Factor C / metabolism*
Vascular Endothelial Growth Factor Receptor-2 / metabolism*
Reg. No./Substance:
0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factor C; EC Endothelial Growth Factor Receptor-2

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