| Expression of USP2-69 in mesangial cells in vivo and in vitro. | |
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MedLine Citation:
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PMID: 20403044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ubiquitin-specific protease 2 (USP2) is a member of a family of de-ubiquitinating enzymes. It may play an important role in the regulation of cell growth and differentiation. It is known that expression of the isoform USP2-69 kD is high in kidney tissue, but its role remains unclear. Mesangial cell proliferation is a prominent element of various types of glomerulonephritides. Therefore, whether USP2 plays a role in mesangial cell proliferation during glomerulonephritides is an interesting question to explore. The purpose of the present study was to evaluate USP2-69 expression in needle biopsies of human kidneys and in cultured rat mesangial cells. On immunohistochemistry USP2-69 was upregulated in some mesangial proliferative glomerulonephritides. The proportion of USP2-69 positive area in the glomeruli was 3.90% in normal kidney, 4.96% in minimal change disease, and 4.39% in membranous glomerulonephritides, while it was 14.84% in IgA nephropathy (IgAN) (mesangial proliferative type), 16.18% in lupus nephritis (LN; diffuse proliferative type) and 15.54% in acute proliferative glomerulonephritides (APGN); the difference of the percentages between IgAN, LN (IV subtype) and APGN and normal kidney were statistically significant (P < 0.05). Additionally, the number of proliferating cell nuclear antigen (PCNA)-positive nuclei in the glomeruli was statistically significantly higher in the various glomerulonephritides than in the normal kidney (P < 0.05). Immunohistochemistry showed that the distribution of the USP2(+) area and PCNA(+) nuclei overlapped in the glomeruli. Treatment with interleukin-1beta for 12 h and 24 h, or with anti-thymocyte serum for 6 h and 12 h resulted in elevated USP2-69 mRNA and protein expression in the rat mesangial cells. Also, PCNA expression increased and p27 expression decreased significantly in the treated mesangial cells. These findings suggest that USP2-69 was upregulated in mesangial cells during mesangial proliferative glomerulonephritides in vivo and in vitro, which may relate to the proliferation of mesangial cells. |
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Authors:
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Suxia Wang; Huijuan Wu; Ye Liu; Jianyong Sun; Zhonghua Zhao; Qi Chen; Muyi Guo; Duan Ma; Zhigang Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pathology international Volume: 60 ISSN: 1440-1827 ISO Abbreviation: Pathol. Int. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-20 Completed Date: 2010-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9431380 Medline TA: Pathol Int Country: Australia |
Other Details:
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Languages: eng Pagination: 184-92 Citation Subset: IM |
Affiliation:
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Department of Pathology and the Key Laboratory of Molecular Medicine (Education Ministry of China), Shanghai Medical College, Fudan University, Shanghai, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Blotting, Western Cell Proliferation* Cells, Cultured Endopeptidases / genetics, metabolism* Glomerulonephritis / genetics, metabolism Glomerulonephritis, IGA / genetics, metabolism Humans Immunohistochemistry Interleukin-1beta / pharmacology Isoenzymes / genetics, metabolism* Lupus Nephritis / genetics, metabolism Male Mesangial Cells / cytology, drug effects, metabolism* RNA, Messenger / genetics, metabolism Rats Reverse Transcriptase Polymerase Chain Reaction Time Factors Up-Regulation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1beta; 0/Isoenzymes; 0/RNA, Messenger; EC 3.4.-/Endopeptidases; EC 3.4.-/USP2 protein, human |
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