Document Detail


Expression of Toll-like receptors 7, 8, and 9 in primary Sjögren's syndrome.
MedLine Citation:
PMID:  20399690     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The objective of this study was to investigate the level of Toll-like receptors (TLRs) 7, 8, and 9 in peripheral mononuclear blood cells (PMBCs) from patients with primary Sjögren's syndrome (pSS) and whether TLR7 and 9 exist in the parotid glands of pSS patients. METHODS: TLR7, 8, and 9 mRNA levels in PMBC from 37 pSS patients and 24 controls were determined using real-time polymerase chain reaction. TLR7- and TLR9-positive cells in parotid glands from 20 pSS patients and 10 controls were observed using immunofluorescence and immunohistochemistry, respectively. Statistical analysis was performed by Student t test. RESULTS: TLR7 and TLR9 mRNA levels were 2.17- and 2.33-fold higher in pSS patients than control subjects, respectively (P < .05), whereas TLR8 levels were not. TLR7- and TLR9-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands and were more abundant than the TLR7- and TLR9-positive cells that were only localized to the ductal epithelial cells of parotid glands (P < .05). CONCLUSION: TLR7 and TLR9 mRNA levels are up-regulated in the PMBC of pSS patients, and TLR7- and TLR9-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of controls.
Authors:
Lingyan Zheng; Zhiyuan Zhang; Chuangqi Yu; Chi Yang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics     Volume:  109     ISSN:  1528-395X     ISO Abbreviation:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508562     Medline TA:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  844-50     Citation Subset:  D; IM    
Copyright Information:
Copyright 2010 Mosby, Inc. All rights reserved.
Affiliation:
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Case-Control Studies
Female
Humans
Immunohistochemistry
Leukocytes, Mononuclear / metabolism
Male
Middle Aged
Parotid Gland / metabolism*
RNA, Messenger / analysis
Reference Values
Sjogren's Syndrome / genetics*,  metabolism
Statistics, Nonparametric
Toll-Like Receptor 7 / genetics,  metabolism
Toll-Like Receptor 8 / genetics,  metabolism
Toll-Like Receptor 9 / genetics,  metabolism
Toll-Like Receptors / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Toll-Like Receptor 7; 0/Toll-Like Receptor 8; 0/Toll-Like Receptor 9; 0/Toll-Like Receptors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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