| Expression of Toll-like receptor 4 on peripheral blood mononuclear cells and its effects on patients with acute myocardial infarction treated with thrombolysis. | |
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MedLine Citation:
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PMID: 21044745 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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BACKGROUND AND AIMS: TLR4 has been shown to mediate inflammation in animal models of myocardial ischemia/reperfusion injury (MI/RI). Here we hypothesized that TLR4 on peripheral blood mononuclear cells (PBMCs) may be involved in the inflammatory response in this type of clinical event. METHODS: Seventy two patients with acute myocardial infarction (AMI) who underwent thrombolysis were assigned into reperfusion group (n = 43) and non-reperfusion group (n = 29) according to recanalization of infarct-related artery (IRA) and 40 healthy volunteers were enrolled in this experiment. Eight mL of venous blood was taken from all patients 0 h before and 2, 6, 12, and 24 h after thrombolysis. Flow cytometry (FCM) was used to detect TLR4 protein expression and real-time quantitative RT-PCR was performed to determine TLR4 mRNA and myeloid differentiation protein-88 (Myd88) mRNA expression. The concentration of tumor necrosis factor-α (TNF-α) in plasma was evaluated using enzyme-linked immunosorbent assay (ELISA). RESULTS: Compared with controls, all detected indicators in AMI patients were upregulated before thrombolysis (p <0.01). After thrombolysis, they were further increased. In reperfusion group, all attained their peaks in earlier hours and the peak values were lower compared with non-reperfusion group. In both cases, either reperfusion or non-perfusion, TLR4 mRNA expression was positively correlated with the levels of Myd88 mRNA (r = 0.886 and 0.694, p <0.01), respectively. CONCLUSIONS: TLR4 expression on PBMCs was markedly elevated in AMI patients either reperfused or non-reperfused. Inflammatory reaction by activated TLR4 in MI/RI in patients may be through TLR4-Myd88-dependent signal pathway. |
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Authors:
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Jun Yang; Lv-ying Jin; Jia-wang Ding; Yong-qin Zhou; Jian Yang; Rui-Yang; Li-li |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of medical research Volume: 41 ISSN: 1873-5487 ISO Abbreviation: Arch. Med. Res. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-11-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9312706 Medline TA: Arch Med Res Country: United States |
Other Details:
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Languages: eng Pagination: 423-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 IMSS. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Cardiology, the First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei Province, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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