| Expression of Sox2 in mouse taste buds and its relation to innervation. | |
| | |
MedLine Citation:
|
PMID: 18379823 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sox2, which encodes an HMG box transcription factor, is known to regulate the differentiation of progenitor cells of the tongue into taste bud cells versus keratinocytes during development. To determine the neural dependence of Sox2 expression, glossopharyngeal nerves of mice were cut bilaterally. In unoperated mice, the expression of Sox2 mRNA and protein was restricted to a subset of taste bud cells and to the epithelium surrounding the taste buds of the circumvallate papillae. During the period of denervation, the taste buds largely disappeared; the taste bud cells and the epithelial cells with Sox2-immunoreactive (IR) nuclei decreased in number and totally disappeared from the epithelium by 16 days after denervation. When regenerated nerve fibers entered the epithelium, Sox2 expression reappeared, first in the epithelial cells, and then in the regenerating taste bud cells. In prenatal mice, Sox2 was expressed in the epithelium of the dorsal surface of circumvallate papillae, in regions into which numerous nerve fibers had entered. The results suggested that Sox2 expression was dependent on gustatory innervation. Sox2-IR cells in the taste buds were also examined by double-immunolabeling for 5-bromo-2'-deoxyuridine and cell-type markers such as cytokeratin 14, neural cell adhesion molecule, inositol 1,4,5-triphosphate receptor 3, and blood group H antigen. Sox2-IR cells were found in the populations of basal cells and of immature and some mature taste bud cells. A large number of Sox2-IR cells were identified as type-I cells, with a few being type-II and type-III cells. |
| | |
Authors:
|
Yuko Suzuki |
Related Documents
:
|
15226643 - Developmental changes of ni(2+) sensitivity and automaticity in nkx2.5-positive cardiac... 16962563 - Cell cycle-dependent regulation of kainate-induced inward currents in microglia. 21833583 - Periostin in fibrillogenesis for tissue regeneration: periostin actions inside and outs... |
Publication Detail:
|
Type: Journal Article Date: 2008-04-01 |
Journal Detail:
|
Title: Cell and tissue research Volume: 332 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-05-12 Completed Date: 2008-08-15 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
|
Languages: eng Pagination: 393-401 Citation Subset: IM |
Affiliation:
|
Division of Histology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan. suzuki@hoku-iryo-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals DNA-Binding Proteins / analysis, genetics, metabolism* Denervation Gene Expression Glossopharyngeal Nerve / physiology HMGB Proteins / analysis, genetics, metabolism* Immunohistochemistry In Situ Hybridization Mice RNA, Messenger / analysis SOXB1 Transcription Factors Taste Buds / embryology, growth & development, metabolism* Transcription Factors / analysis, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/DNA-Binding Proteins; 0/HMGB Proteins; 0/RNA, Messenger; 0/SOXB1 Transcription Factors; 0/Sox2 protein, mouse; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chromosomal regions 22q13 and 3p25 may harbor quantitative trait loci influencing both age at menarc...
Next Document: Ca(2+)/calmodulin-dependent invasion of microvascular endothelial cells of human brain by Escherichi...