Document Detail


Expression of Sox2 in mouse taste buds and its relation to innervation.
MedLine Citation:
PMID:  18379823     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sox2, which encodes an HMG box transcription factor, is known to regulate the differentiation of progenitor cells of the tongue into taste bud cells versus keratinocytes during development. To determine the neural dependence of Sox2 expression, glossopharyngeal nerves of mice were cut bilaterally. In unoperated mice, the expression of Sox2 mRNA and protein was restricted to a subset of taste bud cells and to the epithelium surrounding the taste buds of the circumvallate papillae. During the period of denervation, the taste buds largely disappeared; the taste bud cells and the epithelial cells with Sox2-immunoreactive (IR) nuclei decreased in number and totally disappeared from the epithelium by 16 days after denervation. When regenerated nerve fibers entered the epithelium, Sox2 expression reappeared, first in the epithelial cells, and then in the regenerating taste bud cells. In prenatal mice, Sox2 was expressed in the epithelium of the dorsal surface of circumvallate papillae, in regions into which numerous nerve fibers had entered. The results suggested that Sox2 expression was dependent on gustatory innervation. Sox2-IR cells in the taste buds were also examined by double-immunolabeling for 5-bromo-2'-deoxyuridine and cell-type markers such as cytokeratin 14, neural cell adhesion molecule, inositol 1,4,5-triphosphate receptor 3, and blood group H antigen. Sox2-IR cells were found in the populations of basal cells and of immature and some mature taste bud cells. A large number of Sox2-IR cells were identified as type-I cells, with a few being type-II and type-III cells.
Authors:
Yuko Suzuki
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Publication Detail:
Type:  Journal Article     Date:  2008-04-01
Journal Detail:
Title:  Cell and tissue research     Volume:  332     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-08-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  393-401     Citation Subset:  IM    
Affiliation:
Division of Histology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan. suzuki@hoku-iryo-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA-Binding Proteins / analysis,  genetics,  metabolism*
Denervation
Gene Expression
Glossopharyngeal Nerve / physiology
HMGB Proteins / analysis,  genetics,  metabolism*
Immunohistochemistry
In Situ Hybridization
Mice
RNA, Messenger / analysis
SOXB1 Transcription Factors
Taste Buds / embryology,  growth & development,  metabolism*
Transcription Factors / analysis,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/HMGB Proteins; 0/RNA, Messenger; 0/SOXB1 Transcription Factors; 0/Sox2 protein, mouse; 0/Transcription Factors

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