Document Detail


Expression of Snail is associated with myofibroblast phenotype development in oral squamous cell carcinoma.
MedLine Citation:
PMID:  19198871     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Snail is a regulator of epithelial-mesenchymal transition (EMT) and considered crucial to carcinoma metastasis, myofibroblast transdifferentiation, and fibroblast activation. To investigate the role of Snail in oral squamous cell carcinoma (OSCC), its immunohistochemical expression was analysed in 129 OSCC samples and correlated to nodal metastasis, histological grade, E-cadherin, and alpha smooth-muscle-actin (alpha SMA). The results were compared to findings in 23 basal cell carcinomas (BCC). Additionally, the influence of TGF beta 1 and EGF on Snail, E-cadherin, vimentin, and alpha SMA expression was analysed in two OSCC cell lines. As a result, Snail-positive cells were mainly found in the stroma of the OSCC invasive front without statistically significant correlation to histological grade or nodal metastasis. Snail was co-localised to alpha SMA but not to E-cadherin or cytokeratin and showed a significant correlation to the loss of membranous E-cadherin. All BCCs were Snail negative. In OSCC culture, the growth-factor-mediated EMT-like phenomenon was accompanied by alpha SMA down-regulation. In summary, Snail expression in OSCC is a stromal phenomenon associated with the myofibroblast phenotype and not related to growth-factor-mediated transdifferentiation of the carcinoma cells themselves. Consequently, Snail immunohistochemistry cannot contribute to the prediction of the metastatic potential. Furthermore, stromal Snail expression is suggested to be the result of mutual paracrine interaction of fibro-/myofibroblasts and dedifferentiated carcinoma cells leading to the generation of a special type of carcinoma-associated fibroblasts.
Authors:
Marcus Franz; Karin Spiegel; Claudia Umbreit; Petra Richter; Carolina Codina-Canet; Angela Berndt; Annelore Altendorf-Hofmann; Sven Koscielny; Peter Hyckel; Hartwig Kosmehl; Ismo Virtanen; Alexander Berndt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-06
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  131     ISSN:  1432-119X     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-03-31     Completed Date:  2009-06-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  651-60     Citation Subset:  IM    
Affiliation:
Clinic of Internal Medicine I, University Hospital Jena, 07740, Jena, Germany.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Adult
Aged
Aged, 80 and over
Cadherins / metabolism
Carcinoma, Squamous Cell / metabolism*,  pathology
Epidermal Growth Factor / metabolism
Fibroblasts / metabolism*,  pathology
Humans
Keratins / metabolism
Middle Aged
Mouth Neoplasms / metabolism*,  pathology
Myoblasts / metabolism*,  pathology
Transcription Factors / metabolism*
Transforming Growth Factor beta1 / metabolism
Tumor Markers, Biological / biosynthesis*
Vimentin / metabolism
Chemical
Reg. No./Substance:
0/Actins; 0/Cadherins; 0/Transcription Factors; 0/Transforming Growth Factor beta1; 0/Tumor Markers, Biological; 0/Vimentin; 0/snail family transcription factors; 62229-50-9/Epidermal Growth Factor; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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