Document Detail

Expression of Smad ubiquitin regulatory factor 2 (Smurf2) in rhesus monkey endometrium and placenta during early pregnancy.
MedLine Citation:
PMID:  17189523     Owner:  NLM     Status:  MEDLINE    
Smad ubiquitin regulatory factor 2 (Smurf2) is an E3 ubiquitin ligase that is involved in the Smad-mediated TGF-beta signaling. TGF-beta has been shown to play an important role during normal embryo implantation, but whether Smurf2 is involved in this process has not been reported. This study was first conducted to investigate the expression of Smurf2 transcript and protein in different compartments of the rhesus monkey uteri and placenta during early pregnancy. The results showed that both the cloned partial sequence of Smurf2 gene and the corresponding amino acid residues shared 99% identity with those of human homologs. On day 12 (D12) of pregnancy, strong signals of Smurf2 mRNA were found in basalis glandular epithelium and luminal epithelium, and moderate expressions were detected in functionalis glandular epithelium. During early villi stage and villi placental stage, Smurf2 mRNAs were mainly localized in the placenta villi, trophoblastic column, trophoblastic shell, and basalis glandular epithelium. There appeared strong staining signals in the arterioles on D26 of pregnancy, but faint staining signals on D18 of pregnancy. No specific staining of Smurf2 mRNA was observed in stromal cells and myometrium. The expression pattern of Smurf2 protein was generally similar to that of its mRNA. These results provide the first evidence that Smurf2 may play specific roles in glandular secretion, trophoblastic cell invasion, and placentation through mediating the expression of the related proteins of TGF-beta signaling pathway during early pregnancy.
Qing Yang; Hai-Yan Lin; Hong-Xing Wang; Heng Zhang; Xiaoping Zhang; Hong-Mei Wang; Cheng Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-22
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  55     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-17     Completed Date:  2007-05-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  453-60     Citation Subset:  IM    
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Haidian District, Beijing, China.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Endometrium / metabolism*
Macaca mulatta
Molecular Sequence Data
Placenta / metabolism*
Pregnancy, Animal / metabolism*
RNA, Messenger / biosynthesis
Sequence Homology, Amino Acid
Time Factors
Ubiquitin-Protein Ligases / biosynthesis*,  genetics
Reg. No./Substance:
0/RNA, Messenger; EC protein, human; EC Ligases

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