Document Detail


Expression of Slit2 and Robo1 after traumatic lesions of the rat spinal cord.
MedLine Citation:
PMID:  19783284     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
We have used semi-quantitative RT-PCR, Western blot, and immunofluorescence imaging approaches to detect the expression levels of Slit2 and its receptor Robo1 in the rat spinal cord after traumatic lesions. Our results revealed that both the mRNA and protein levels of Slit2 were up-regulated in the injured spinal cord. The Slit2 expression level was increased at day 7 until day 14, and then returned to normal level at day 21 after injury. A double-immunolabelling study showed that Slit2 and neurofilament (NF) proteins were both localized in neurons of spinal corda cinerea. Slit2 immunopositivity was detected in neuronal plasma membranes but not in the axonal fibers. In contrast, the immunolabelling of Robo1 in the normal spinal cord was at a low level, mostly in the neurons of spinal corda cinerea, and remained unchanged at all time points following spinal cord injury (SCI). The regulation levels of Slit2 and Robo1 after traumatic lesions in the rat spinal cord are different. Our results indicate that Slit2-Robo1 might not be involved in the inhibitory environment after SCI.
Authors:
Jin-Bo Liu; Yu-Qin Jiang; Ai-Hua Gong; Zhi-Jian Zhang; Qian Jiang; Xiang-Ping Chu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-23
Journal Detail:
Title:  Acta histochemica     Volume:  113     ISSN:  1618-0372     ISO Abbreviation:  Acta Histochem.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  43-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2009 Elsevier GmbH. All rights reserved.
Affiliation:
Department of Orthopedics, The Third Affiliated Hospital, Suzhou University, Changzhou, China. jsczljb@yahoo.com.cn
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