| Expression of Schwann cell markers by mammalian neural crest cells in vitro. | |
| | |
MedLine Citation:
|
PMID: 2806124 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
During embryonic development, neural crest cells differentiate into a wide variety of cell types including Schwann cells of the peripheral nervous system. In order to establish when neural crest cells first start to express a Schwann cell phenotype immunocytochemical techniques were used to examine rat premigratory neural crest cell cultures for the presence of Schwann cell markers. Cultures were fixed for immunocytochemistry after culture periods ranging from 1 to 24 days. Neural crest cells were identified by their morphology and any neural tube cells remaining in the cultures were identified by their epithelial morphology and immunocytochemically. As early as 1 to 2 days in culture, approximately one third of the neural crest cells stained with m217c, a monoclonal antibody that appears to recognize the same antigen as rat neural antigen-1 (RAN-1). A similar proportion of cells were immunoreactive in cultures stained with 192-IgG, a monoclonal antibody that recognizes the rat nerve growth factor receptor. The number of immunoreactive cells increased with time in culture. After 16 days in culture, nests of cells, many of which had a bipolar morphology, were present in the area previously occupied by neural crest cells. The cells in the nests were often associated with neurons and were immunoreactive for m217c, 192-IgG and antibody to S-100 protein and laminin, indicating that the cells were Schwann cells. At all culture periods examined, neural crest cells did not express glial fibrillary acidic protein. These results demonstrate that cultured premigratory neural crest cells express early Schwann cell markers and that some of these cells differentiate into Schwann cells. These observations suggest that some neural crest cells in vivo may be committed to forming Schwann cells and will do so provided that they then proceed to encounter the correct environmental cues during embryonic development. |
| | |
Authors:
|
L C Smith-Thomas; J W Fawcett |
Related Documents
:
|
10761114 - Factors controlling lineage specification in the neural crest. 12882174 - Expansion and neural differentiation of embryonic stem cells in adherent and suspension... 9758704 - A single rostrocaudal colonization of the rodent intestine by enteric neuron precursors... 9878714 - Two groups of th-like immunoreactive neurons in the frog (rana pipiens) retina. 22743374 - Lipopolysaccharide effects on the proliferation of nrk52e cells via alternations in gap... 2138574 - Dissemination of endometrial cells during carbon dioxide hysteroscopy and chromotubatio... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 105 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 1989 Feb |
Date Detail:
|
Created Date: 1989-12-21 Completed Date: 1989-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 251-62 Citation Subset: IM |
Affiliation:
|
Physiological Laboratory, Cambridge, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Markers Cells, Cultured Epithelium Immunohistochemistry Melanocytes Neural Crest / physiology* Rats Rats, Inbred Strains Schwann Cells / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Fertilization and ooplasmic movements in the ascidian egg.
Next Document: Expression and role of E- and P-cadherin adhesion molecules in embryonic histogenesis. I. Lung epith...