Document Detail

Expression of the Saccharomyces cerevisiae PIS gene and synthesis of phosphatidylinositol in Escherichia coli.
MedLine Citation:
PMID:  2844726     Owner:  NLM     Status:  MEDLINE    
Expression of the Saccharomyces cerevisiae PIS gene encoding phosphatidylinositol synthase in Escherichia coli was achieved by inserting its coding sequence into lacZ on pUC8. The fused gene encoded a phosphatidylinositol synthase whose amino-terminal three amino acids had been replaced by the amino-terminal five amino acids of E. coli beta-galactosidase. E. coli cells bearing this recombinant plasmid produced a significant level of phosphatidylinositol synthase in the presence of a lacZ inducer, isopropylthio-beta-D-galactopyranoside. When the culture medium was supplemented with myo-inositol and isopropylthio-beta-D-galactopyranoside, the cells accumulated a substantial amount of phosphatidylinositol in their membranes. When a saturating level of myo-inositol was added, phosphatidylinositol constituted about 4% of the total phospholipids. Phosphatidylinositol accumulation occurred at the expense of phosphatidylglycerol. The ratio of phosphatidylethanolamine to total acidic phospholipids remained constant. The growth rate of phosphatidylinositol-containing E. coli cells did not differ significantly from that of cells with the normal phospholipid composition.
J Nikawa; T Kodaki; S Yamashita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  170     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-11-14     Completed Date:  1988-11-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4727-31     Citation Subset:  IM    
Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan.
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MeSH Terms
CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
Cloning, Molecular
Escherichia coli / genetics*,  growth & development,  metabolism
Gene Expression Regulation
Membrane Lipids / biosynthesis
Phosphatidylglycerols / biosynthesis
Phosphatidylinositols / biosynthesis*
Phosphotransferases / genetics*
Saccharomyces cerevisiae / genetics*
Transferases (Other Substituted Phosphate Groups)*
Reg. No./Substance:
0/Membrane Lipids; 0/Phosphatidylglycerols; 0/Phosphatidylinositols; EC 2.7.-/Phosphotransferases; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups); EC 3-Phosphatidyltransferase

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