| Expression of the Saccharomyces cerevisiae PIS gene and synthesis of phosphatidylinositol in Escherichia coli. | |
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MedLine Citation:
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PMID: 2844726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Expression of the Saccharomyces cerevisiae PIS gene encoding phosphatidylinositol synthase in Escherichia coli was achieved by inserting its coding sequence into lacZ on pUC8. The fused gene encoded a phosphatidylinositol synthase whose amino-terminal three amino acids had been replaced by the amino-terminal five amino acids of E. coli beta-galactosidase. E. coli cells bearing this recombinant plasmid produced a significant level of phosphatidylinositol synthase in the presence of a lacZ inducer, isopropylthio-beta-D-galactopyranoside. When the culture medium was supplemented with myo-inositol and isopropylthio-beta-D-galactopyranoside, the cells accumulated a substantial amount of phosphatidylinositol in their membranes. When a saturating level of myo-inositol was added, phosphatidylinositol constituted about 4% of the total phospholipids. Phosphatidylinositol accumulation occurred at the expense of phosphatidylglycerol. The ratio of phosphatidylethanolamine to total acidic phospholipids remained constant. The growth rate of phosphatidylinositol-containing E. coli cells did not differ significantly from that of cells with the normal phospholipid composition. |
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Authors:
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J Nikawa; T Kodaki; S Yamashita |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 170 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1988 Oct |
Date Detail:
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Created Date: 1988-11-14 Completed Date: 1988-11-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4727-31 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase Cloning, Molecular Escherichia coli / genetics*, growth & development, metabolism Gene Expression Regulation Membrane Lipids / biosynthesis Phosphatidylglycerols / biosynthesis Phosphatidylinositols / biosynthesis* Phosphotransferases / genetics* Saccharomyces cerevisiae / genetics* Transferases (Other Substituted Phosphate Groups)* |
| Chemical | |
Reg. No./Substance:
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0/Membrane Lipids; 0/Phosphatidylglycerols; 0/Phosphatidylinositols; EC 2.7.-/Phosphotransferases; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups); EC 2.7.8.11/CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase |
| Comments/Corrections | |
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