| Expression and Response of Acid-Sensing Ion Channels (ASICs) in Urinary Bladder to Cyclophosphamide (CYP)-Induced Cystitis. | |
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MedLine Citation:
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PMID: 20164155 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The expression of acid sensing ion channels (ASICs) isoforms, ASIC1, ASIC2a and ASIC3, were examined in the urinary bladder after cyclophosphamide (CYP)-induced cystitis of varying duration (4 hours (h), 48 h, chronic). Immunohistochemical, western blotting and quantitative PCR approaches were used to evaluate channel expression and effects of CYP-induced cystitis in whole urinary bladder and split bladder preparations from control (no inflammation) and CYP-treated rats. Quantitative polymerase chain reaction demonstrated significant (p </= 0.01) increases in ASIC2a and ASIC3 transcripts with CYP-induced cystitis (48 h and chronic) in the urothelium but no changes (e.g., ASIC3) or modest changes (e.g., ASIC2a) were demonstrated in detrusor smooth muscle with CYP-induced cystitis. ASIC1 mRNA expression was not affected in urothelium or detrusor by CYP-induced cystitis. Immunohistochemistry for ASIC2a and ASIC3 protein expression revealed significant (p </= 0.01) increases in ASIC-IR in the urothelium and suburothelial plexus with CYP-induced cystitis at all time points examined. Western blotting for ASIC2a and ASIC3 protein expression were complementary and revealed significant (p </= 0.01) increases in ASIC-IR. For the first time, these studies demonstrate that urinary bladder inflammation induced by CYP alters ASIC2a and ASIC3 expression in the urinary bladder; ASIC1 transcript expression is not altered by CYP-induced cystitis. Future studies are necessary to determine ASIC isoform contributions to micturition reflexes in control and inflamed urinary bladder. Key words: urothelium, suburothelial nerve plexus, ASIC2a, ASIC3, Q-PCR. |
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Authors:
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Kimberly Corrow; Beatrice M Girard; Margaret A Vizzard |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-2-17 |
Journal Detail:
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Title: American journal of physiology. Renal physiology Volume: - ISSN: 1522-1466 ISO Abbreviation: - Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-2-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of Vermont College of Medicine. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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