| Expression of protein kinase C α and the MZF-1 and Elk-1 transcription factors in human breast cancer cells. | |
| | |
MedLine Citation:
|
PMID: 22242952 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recently, our research into hepatocellular carcinoma (HCC) has shown that the transcription factors Myeloid Zinc Finger-1 (MZF-1) and Ets-like-protein 1 are related to protein kinase C alpha (PKCα) expression. The purpose of this study was to determine the correlation of the expression of PKCαwith the expressions of Elk-1 and MZF-1 in various differentiated breast cancer cell lines: MDA- MB-231, Hs57BT, SKBR3, MDA-MB-468 and MCF-7. The malignant potential in the five lines of breast cancer cells was examined by using a cell proliferation/migration/invasion assay and the protein and mRNA levels of PKCα, ElK-1 and MZF-1 were examined by Western blot and RT-PCR analysis, re- spectively. The results showed that there were obvious signs of migration and invasion of cells in MDA- MB-231 and Hs57BT cells, little signs of cell migration and invasion in MDA-MB-468 cells, and no sign in SKBR3 and MCF-7 cells. Moreover, the highest expression levels of PKCα, Elk-1 and MZF-1 were also observed in MDA-MB-231 and Hs57BT cells when compared to the other breast cancer cell lines. These findings confirm that elevated expression of PKCαin breast cancer cells may be correlated with the potential of cell migration and invasion, and suggest an association between the expression of PKCα and the expression of the transcription factors Elk-1 and MZF-1. |
| | |
Authors:
|
Chia-Herng Yue; Yung-Wei Chiu; Jai-Nien Tung; Bor-Show Tzang; Jiuan-Jen Shiu; Wen-Hung Huang; Jer-Yuh Liu; Jin-Ming Hwang |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Chinese journal of physiology Volume: 55 ISSN: 0304-4920 ISO Abbreviation: Chin J Physiol Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-01-16 Completed Date: 2012-04-19 Revised Date: 2012-06-25 |
Medline Journal Info:
|
Nlm Unique ID: 7804502 Medline TA: Chin J Physiol Country: China (Republic : 1949- ) |
Other Details:
|
Languages: eng Pagination: 31-6 Citation Subset: IM |
Affiliation:
|
Department of Surgery, Tungs' Taichung Metro Harbor Hospital, Taichung, Taiwan, Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Breast Neoplasms
/
enzymology* Cell Line, Tumor Cell Movement Cell Proliferation Female Humans Kruppel-Like Transcription Factors / metabolism* Neoplasm Invasiveness Protein Kinase C-alpha / metabolism* ets-Domain Protein Elk-1 / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/ELK1 protein, human; 0/Kruppel-Like Transcription Factors; 0/MZF1 protein, human; 0/ets-Domain Protein Elk-1; EC 2.7.11.13/PRKCA protein, human; EC 2.7.11.13/Protein Kinase C-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Attenuation of the extract from moringa oleifera on monocrotaline-induced pulmonary hypertension in ...
Next Document: "Anti-Inflammatory Effects of Probiotic Lactobacillus paracasi on Ventricles of BALB/C Mice Treated ...