| Expression of proteasomal immunosubunit beta1i is dysregulated in inflammatory infiltrates of minor salivary glands in Sjogren's syndrome. | |
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MedLine Citation:
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PMID: 19833746 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Minor salivary gland specimens were analyzed to investigate dysregulation of the proteasome system in patients with Sj??gren's syndrome (SS) and patients with sicca syndrome. METHODS: Labial biopsy specimens from 17 patients with SS and 11 patients with non-autoimmunesicca syndrome were analyzed by immunohistochemistry for expression of the inducible proteasomal subunits ss1i, ss2i, and ss5i. The infiltrating subsets of lymphocytes were characterized using immunofluorescence stainings against the cell-surface markers CD20 and CD27. Two-dimensional electrophoresis and immunoblotting were used for detection of the proteasomal subunits ss1 and ss1i in peripheral blood monocyte cells. Gene expression of the constitutive subunits ss1, ss2, and ss5 and the corresponding inducible subunits ss1i, ss2i, and ss5i was further investigated at the mRNA level in small lip biopsies using real-time polymerase chain reaction. RESULTS: The expression of ss1i in infiltrating and peripheral immune cells was altered in patients with SS compared to patients with non-autoimmune sicca syndrome and healthy controls. No significant differences were found in ss2i and ss5i expression between the same groups in small lip biopsies. Chisholm-Mason grade and ss1i expression were found to be inversely correlated (Spearman r = -0.461, p = 0.014). The phenotype and distribution of the lymphocytic infiltrate showed no differences between patients with primary and secondary SS regardless of ss1i expression. CONCLUSION: The proteasomal ss1i subunit is dysregulated in peripheral white blood cells and in inflammatory infiltrates of minor salivary glands in patients with SS. |
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Authors:
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Lars Morawietz; Lorena Martinez-Gamboa; Sonja Scheffler; Gert Hausdorf; Anja Dankof; Ulrike Kuckelkorn; Thomas Doerner; Karl Egerer; Gerd-R Burmester; Denise L Faustman; Eugen Feist |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-15 |
Journal Detail:
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Title: The Journal of rheumatology Volume: 36 ISSN: 0315-162X ISO Abbreviation: J. Rheumatol. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-07 Completed Date: 2010-02-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7501984 Medline TA: J Rheumatol Country: Canada |
Other Details:
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Languages: eng Pagination: 2694-703 Citation Subset: IM |
Affiliation:
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Department of Rheumatology and Clinical Immunology, Institute of Pathology, and Institute of Biochemistry, Charit??-Universit??tsmedizin Berlin, Free University and Humboldt University of Berlin, Berlin, Germany. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Animals B-Lymphocyte Subsets / immunology B-Lymphocytes / immunology Biopsy Cysteine Endopeptidases / genetics, metabolism* Female Humans Lip / cytology, pathology Male Middle Aged Molecular Sequence Data Protein Subunits / genetics, immunology* Salivary Glands, Minor* / immunology, metabolism, pathology Sjogren's Syndrome* / immunology, metabolism, pathology T-Lymphocyte Subsets / immunology T-Lymphocytes / immunology Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Protein Subunits; 144416-78-4/LMP-2 protein; EC 3.4.22.-/Cysteine Endopeptidases |
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