Document Detail

Expression of Protease Activated Receptor-1 in Chronic Periodontitis.
MedLine Citation:
PMID:  25058238     Owner:  NLM     Status:  Publisher    
Background: Protease activated receptor 1 (PAR1) activation by thrombin may play a role in repair and homeostasis of periodontal tissues. The main objective of this study was to investigate PAR1 expression in periodontitis patients, before and after non-surgical periodontal treatment, and to associate its expression with the presence of inflammatory biomarkers, and PAR2 expression. Methods: Gingival crevicular fluid (GCF) samples and clinical parameters consisting of measuring probing depths (PD), clinical attachment level (CAL), bleeding on probing (BOP), and gingival (GI) and plaque index (PI) were collected from periodontally healthy and moderate chronic periodontitis patients before and 6 weeks after periodontal non-surgical treatment. PAR1 and PAR2 mRNA at the GCF were evaluated by qPCR. Flow cytometry analysis identified the GCF PAR1-expressing cells. GCF inflammatory biomarkers were also determined. Results: Clinical parameters were significantly improved after therapy (p < 0.01). The qPCR analysis showed that before therapy, PAR1 mRNA levels in chronic periodontitis were similar to controls. Periodontal treatment led to increased PAR1 expression in chronic periodontitis (p < 0.05). PAR1 expression was inversely correlated to PAR2 expression, and with interleukin (IL)-6, IL-8, tumor necrosis factor α, interferon-γ, and matrix metalloproteinase-2 levels. Conclusion: In conclusion, we have shown that periodontal treatment results in PAR1 over-expression in the GCF, and that PAR1 expression is associated with decreased expression of inflammatory biomarkers and inversely correlated to PAR2 expression in the GCF. Therefore, our data suggest the importance of PAR1 mediating the known anabolic actions of thrombin in the periodontium.
Henrique Aparecido Bueno da Silva; Vanessa Tubero Euzebio Alves; Luis Carlos Spolidório; João Batista César Neto; Rosangela Santos Eichler; Maria Helena Catelli de Carvalho; Marinella Holzhausen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-24
Journal Detail:
Title:  Journal of periodontology     Volume:  -     ISSN:  1943-3670     ISO Abbreviation:  J. Periodontol.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-13     Citation Subset:  -    
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