| Expression profiling of a novel calcium-dependent protein kinase gene, LeCPK2, from tomato (Solanum lycopersicum) under heat and pathogen-related hormones. | |
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MedLine Citation:
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PMID: 19897910 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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A full-length cDNA LeCPK2 (GenBank GQ205414) from tomato (Solanum lycopersicum) encoding a calcium-dependent protein kinase (CDPK) was cloned by in silico cloning using NtCPK5 (AY971376) as a virtual probe. The deduced amino acid sequence of LeCPK2 contained the kinase, autoinhibitory, and calmodulin-like domains typical of CDPKs. Expression profiling indicated that LeCPK2 expressed predominantly in flowers and responded divergently to heat and cold stress, in which obvious mRNA accumulation was detected at 4 h under 42 degrees C stress, but no change in LeCPK2 mRNA levels was observed in 6 h at 4 degrees C. Mechanical wounding and phytohormones including ethylene, methyl jasmonate, and salicylic acid were also observed to arouse the expression of LeCPK2 in a similar pattern. mRNA accumulation was enhanced at 30 min and reached a maximum at 3 h, followed by a decrease to the normal level. All the results suggest that LeCPK2 is a novel versatile isoform of tomato CDPKs. |
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Authors:
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Wen-Jun Chang; Huo-Sheng Su; Wei-Jing Li; Zhi-Li Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 73 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 2427-31 Citation Subset: IM |
Affiliation:
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Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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