Document Detail


Expression of Prnp mRNA (prion protein gene) in mouse spermatogenic cells.
MedLine Citation:
PMID:  15514463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Prnp (prion protein) gene, which encodes a soluble protein anchored to the cell surface by glycosylphosphatidylinositol (GPI), might be involved in cell-to-cell interaction. The expression of Prnp is strongly observed not only in the brain, but also in non-neuronal tissues. In order to examine the Prnp expression sites in mouse testes, we carried out Northern blot and in situ hybridization analyses. By Northern blot analysis, two kinds of Prnp transcripts (major band of 2.2 kb, and minor band of 1.1 kb) were detected in testes. The 2.2-kb transcript was observed in testes throughout the postnatal development, whereas the 1.1-kb transcript was observed in testes from 2 to 70 weeks old. In situ hybridization analysis showed that the positive signals for Prnp mRNAs were predominantly observed in spermatogenic cells, but not in somatic cells such as Sertoli cells, Leydig cells and peritubular myoid cells. The signals were observed moderately in spermatogonia, and strongly in spermatocytes and round spermatids, but not in elongate spermatids and spermatozoa. These results suggest that Prnp may be involved in germ cell differentiation during mammalian spermatogenesis.
Authors:
Masahiko Fujisawa; Yoshiakira Kanai; Sang-Yoon Nam; Seishi Maeda; Nobuaki Nakamuta; Kiyoshi Kano; Masamichi Kurohmaru; Yoshihiro Hayashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  50     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-10-29     Completed Date:  2005-03-04     Revised Date:  2006-11-28    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  565-70     Citation Subset:  IM    
Affiliation:
Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Japan. msf34jsw@mail.vm.a.u-tokyo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Amyloid / genetics*
Animals
Blotting, Northern
Cell Differentiation / physiology
In Situ Hybridization
Male
Mice
Mice, Inbred C57BL
Prions
Protein Precursors / genetics*
RNA, Messenger / analysis
Spermatogenesis / physiology*
Spermatogonia / physiology*
Chemical
Reg. No./Substance:
0/Amyloid; 0/Prions; 0/Prnp protein, mouse; 0/Protein Precursors; 0/RNA, Messenger

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