| Expression of Pin1, a peptidyl-prolyl isomerase, in the ovaries of eCG/hCG-treated immature female mice. | |
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MedLine Citation:
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PMID: 16394625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protein phosphorylation on certain serine or threonine residues preceding proline (Ser/Thr-Pro) is a pivotal signaling mechanism in diverse cellular processes. Pin1 is a highly conserved enzyme that isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby inducing conformational changes. Although much protein is phosphorylated in the ovary, the role of Pin1 in the ovary is still unknown. The purpose of this study is to investigate the effects of gonadotropins on protein and mRNA expression of Pin1 in mice ovaries. Quantitative PCR analysis showed that the expression of Pin1 mRNA significantly increased in the ovaries of equine chorionic gonadotropin (eCG)-treated mice compared with those of untreated mice (P<0.05). However, human chorionic gonadotropin (hCG) attenuated the expression of Pin1 mRNA increased by eCG. The protein level of Pin1 showed the same tendency as the expression of mRNA. The mRNA expression of E2F transcription factor, which controlled the expression of Pin1, was significantly decreased in the eCG-treated ovaries compared with the controls (P<0.05). These observations suggest that gonadotropins may regulate the expression of Pin1 without E2F transcription factor, indicating that Pin1 might be an important factor for protein signal transduction during follicular development. |
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Authors:
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Takashi Shimizu; Hirotada Akiyama; Yasuyuki Abe; Hiroshi Sasada; Eimei Sato; Akio Miyamoto; Takafumi Uchida |
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Publication Detail:
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Type: Journal Article Date: 2005-12-28 |
Journal Detail:
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Title: The Journal of reproduction and development Volume: 52 ISSN: 0916-8818 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-05-02 Completed Date: 2006-06-12 Revised Date: 2006-10-07 |
Medline Journal Info:
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Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: Japan |
Other Details:
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Languages: eng Pagination: 287-91 Citation Subset: IM |
Affiliation:
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Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Japan. shimizut@obihiro.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Chorionic Gonadotropin / pharmacology* E2F Transcription Factors / metabolism Female Glycoprotein Hormones, alpha Subunit / pharmacology* Gonadotropins / metabolism Mice Mice, Inbred ICR Models, Statistical Ovary / metabolism* Peptidylprolyl Isomerase / biosynthesis*, chemistry, metabolism Phosphorylation Polymerase Chain Reaction RNA / metabolism RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Serine / chemistry Signal Transduction Threonine / chemistry Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/E2F Transcription Factors; 0/Glycoprotein Hormones, alpha Subunit; 0/Gonadotropins; 0/NIMA-interacting peptidylprolyl isomerase; 0/RNA, Messenger; 56-45-1/Serine; 63231-63-0/RNA; 72-19-5/Threonine; EC 5.2.1.8/Peptidylprolyl Isomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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