Document Detail


Expression of PEP-19 inhibits apoptosis in PC12 cells.
MedLine Citation:
PMID:  11117479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PEP-19 is a calmodulin-regulatory protein found specifically within neurons, though cellular functions of this protein have not been determined. In an effort to define potential effects of PEP-19, PC12 cell lines expressing this protein were generated and subjected to apoptotic stimuli. As measured by LDH release, cell death in PEP-19 expressing cells was 2- to 5-fold less following u.v. irradiation, and 2- to 4-fold less following staurosporine treatment than controls. Additionally, PEP-19-expressing cells displayed decreased DNA ladder formation, chromatin and condensation, caspase activation following staurosporine treatment. Overall, these results demonstrate that PEP-19 can inhibit apoptotic processes in PC12 cells, suggesting a potential regulatory mechanism for pathways leading to cell death.
Authors:
J A Erhardt; J J Legos; R A Johanson; J R Slemmon; X Wang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroreport     Volume:  11     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-14     Completed Date:  2001-03-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  3719-23     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
Enzyme Activation
L-Lactate Dehydrogenase / metabolism
Nerve Tissue Proteins / metabolism,  physiology*
Neurons / drug effects,  physiology*,  radiation effects
PC12 Cells
Rats
Staurosporine / pharmacology
Ultraviolet Rays
Grant Support
ID/Acronym/Agency:
NS33299/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Pcp4 protein, rat; 62996-74-1/Staurosporine; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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