Document Detail

Expression of PEP-19 inhibits apoptosis in PC12 cells.
MedLine Citation:
PMID:  11117479     Owner:  NLM     Status:  MEDLINE    
PEP-19 is a calmodulin-regulatory protein found specifically within neurons, though cellular functions of this protein have not been determined. In an effort to define potential effects of PEP-19, PC12 cell lines expressing this protein were generated and subjected to apoptotic stimuli. As measured by LDH release, cell death in PEP-19 expressing cells was 2- to 5-fold less following u.v. irradiation, and 2- to 4-fold less following staurosporine treatment than controls. Additionally, PEP-19-expressing cells displayed decreased DNA ladder formation, chromatin and condensation, caspase activation following staurosporine treatment. Overall, these results demonstrate that PEP-19 can inhibit apoptotic processes in PC12 cells, suggesting a potential regulatory mechanism for pathways leading to cell death.
J A Erhardt; J J Legos; R A Johanson; J R Slemmon; X Wang
Related Documents :
1965839 - Characterization of functional nerve growth factor-receptors in a cns glial cell line: ...
7766849 - Ngf-dependent neurotrophic-like effects of af102b, an m1 muscarinic agonist, in pc12m1 ...
11129979 - New structural analogues of tubulozole induce apoptosis, [ca2+]i modifications and cyto...
1646299 - Phospholipid-mediated delivery of anti-gap-43 antibodies into neuroblastoma cells preve...
2351099 - Expression of 25-hydroxyvitamin d3-24-hydroxylase activity in caco-2 cells. an in vitro...
9262149 - Differentiated b104 neuroblastoma cells are a high-resolution assay for micropatterned ...
24265019 - Modulation of retinal müller cells by complement receptor c5ar.
19660579 - Microsphere-based scaffolds for cartilage tissue engineering: using subcritical co(2) a...
59709 - Cell line-specific cell surface components of hypotetraploid ascites mammary carcinoma ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroreport     Volume:  11     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-14     Completed Date:  2001-03-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  3719-23     Citation Subset:  IM    
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Apoptosis / drug effects*
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
Enzyme Activation
L-Lactate Dehydrogenase / metabolism
Nerve Tissue Proteins / metabolism,  physiology*
Neurons / drug effects,  physiology*,  radiation effects
PC12 Cells
Staurosporine / pharmacology
Ultraviolet Rays
Grant Support
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Pcp4 protein, rat; 62996-74-1/Staurosporine; EC Dehydrogenase; EC Protein Kinase Type 2; EC Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Deviant neurophysiological asymmetry in children with language impairment.
Next Document:  Neuronal ageing in long-term cultures: alterations of Ca2+ homeostasis.