| Expression of PEP-19 inhibits apoptosis in PC12 cells. | |
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MedLine Citation:
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PMID: 11117479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PEP-19 is a calmodulin-regulatory protein found specifically within neurons, though cellular functions of this protein have not been determined. In an effort to define potential effects of PEP-19, PC12 cell lines expressing this protein were generated and subjected to apoptotic stimuli. As measured by LDH release, cell death in PEP-19 expressing cells was 2- to 5-fold less following u.v. irradiation, and 2- to 4-fold less following staurosporine treatment than controls. Additionally, PEP-19-expressing cells displayed decreased DNA ladder formation, chromatin and condensation, caspase activation following staurosporine treatment. Overall, these results demonstrate that PEP-19 can inhibit apoptotic processes in PC12 cells, suggesting a potential regulatory mechanism for pathways leading to cell death. |
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Authors:
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J A Erhardt; J J Legos; R A Johanson; J R Slemmon; X Wang |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neuroreport Volume: 11 ISSN: 0959-4965 ISO Abbreviation: Neuroreport Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-12-14 Completed Date: 2001-03-01 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9100935 Medline TA: Neuroreport Country: England |
Other Details:
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Languages: eng Pagination: 3719-23 Citation Subset: IM |
Affiliation:
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Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Calcium-Calmodulin-Dependent Protein Kinase Type 2 Calcium-Calmodulin-Dependent Protein Kinases / metabolism Enzyme Activation L-Lactate Dehydrogenase / metabolism Nerve Tissue Proteins / metabolism, physiology* Neurons / drug effects, physiology*, radiation effects PC12 Cells Rats Staurosporine / pharmacology Ultraviolet Rays |
| Grant Support | |
ID/Acronym/Agency:
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NS33299/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nerve Tissue Proteins; 0/Pcp4 protein, rat; 62996-74-1/Staurosporine; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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