Document Detail


Expression of Nox1 in 3T3 cells increases cellular acid production but not proton conductance.
MedLine Citation:
PMID:  17234149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of the NADPH oxidase homolog 1 (Nox1) in plasma membrane H(+) conductance and cellular H(+) production was investigated in 3T3 cells stably expressing Nox1 (Nox1 3T3) compared to vector-expressing control cells (mock 3T3). In whole cell patch clamp experiments both Nox1 and mock 3T3 expressed a similar H(+) conductance (Nox1 3T3, 13.2+/-8.6 pS/pF; mock 3T3, 16.6+/-13.4 pS/pF) with a number of similar characteristics (e.g., current-voltage relations, current activation kinetics, Zn(2+)-sensitivity). When the intracellular pH of cells was alkalinized with NH(4)Cl, rates of intracellular acidification were significantly higher in Nox1 3T3 compared to mock 3T3. Nox1 3T3 showed a time course of acidification that followed a double-exponential function with a fast and a slow component of, on average, tau=165 s and 1780 s, whereas mock 3T3 showed only a single slow tau of 1560 s. Expression of Nox1 also caused cells to acidify the extracellular medium at higher rates than control cells; Nox1 3T3 released 96+/-19 fmol h(-1)cell(-1) of acid equivalents compared to 19+/-12 fmol h(-1)cell(-1) in mock 3T3. These data show that expression of Nox1 results in a mechanism that has the capacity to rapidly acidify the cytosol and generate significant amounts of acid. No significant effect of Nox1 expression on the plasma membrane H(+) conductance was found.
Authors:
Vincent Gaggioli; Christian Schwarzer; Horst Fischer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-12-06
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  459     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-14     Completed Date:  2007-05-18     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  189-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cell Membrane / chemistry*,  physiology*
Cell Membrane Permeability / physiology*
Electric Conductivity
Hydrogen-Ion Concentration
Mice
NADH, NADPH Oxidoreductases / metabolism*
Protons*
Grant Support
ID/Acronym/Agency:
HL071829/HL/NHLBI NIH HHS; R01 HL071829/HL/NHLBI NIH HHS; R01 HL071829-01/HL/NHLBI NIH HHS; R01 HL071829-02/HL/NHLBI NIH HHS; R01 HL071829-03/HL/NHLBI NIH HHS; R01 HL071829-04/HL/NHLBI NIH HHS; R01 HL086323/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Protons; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.99.-/NADPH oxidase 1
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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