Document Detail


Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice.
MedLine Citation:
PMID:  8643575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alternatives to cell culture systems for production of recombinant proteins could make very safe vaccines at a lower cost. We have used genetically engineered plants for expression of candidate vaccine antigens with the goal of using the edible plant organs for economical delivery of oral vaccines. Transgenic tobacco and potato plants were created that express the capsid protein of Norwalk virus, a calicivirus that causes epidemic acute gastroenteritis in humans. The capsid protein could be extracted from tobacco leaves in the form of 38-nm Norwalk virus-like particles. Recombinant Norwalk virus-like particle (rNV) was previously recovered when the same gene was expressed in recombinant baculovirus-infected insect cells. The capsid protein expressed in tobacco leaves and potato tubers cosedimented in sucrose gradients with insect cell-derived rNV and appeared identical to insect cell-derived rNV on immunoblots of SDS/polyacrylamide gels. The plant-expressed rNV was orally immunogenic in mice. Extracts of tobacco leaf expressing rNV were given to CD1 mice by gavage, and the treated mice developed both serum IgG and secretory IgA specific for rNV. Furthermore, when potato tubers expressing rNV were fed directly to mice, they developed serum IgG specific for rNV. These results indicate the potential usefulness of plants for production and delivery of edible vaccines. This is an appropriate technology for developing countries where vaccines are urgently needed.
Authors:
H S Mason; J M Ball; J J Shi; X Jiang; M K Estes; C J Arntzen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  93     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-07-17     Completed Date:  1996-07-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5335-40     Citation Subset:  IM    
Affiliation:
Plant Biotechnology Program, Albert Alkek Institute of Biosciences and Technology, Texas A & M University, Houston, 77030-3303, USA.
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Animals
Antibodies, Viral / biosynthesis
Antibody Formation
Base Sequence
Capsid / biosynthesis*,  immunology*
Cell Line
DNA Primers
Mice
Mice, Inbred Strains
Molecular Sequence Data
Norwalk virus / genetics,  immunology*,  metabolism
Plants, Genetically Modified
Plants, Toxic
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
Solanum tuberosum / metabolism
Spodoptera
Tobacco / metabolism
Transfection
Vaccines, Synthetic / administration & dosage*
Viral Vaccines / administration & dosage*
Grant Support
ID/Acronym/Agency:
AI 36519/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/DNA Primers; 0/Vaccines, Synthetic; 0/Viral Vaccines
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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