Document Detail


Expression of NO scavenging hemoglobin is involved in the timing of bolting in Arabidopsis thaliana.
MedLine Citation:
PMID:  18034356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants contain three classes of hemoglobin genes of which two, class 1 and class 2, have a structure similar to classical vertebrate globins. We investigated the effect of silencing the class 1 non-symbiotic hemoglobin gene, GLB1, and the effect of overexpression of GLB1 or the class 2 non-symbiotic hemoglobin gene, GLB2, in Arabidopsis thaliana. Lines with GLB1 silencing had a significant delay of bolting and after bolting, shoots reverted to the rosette vegetative phase by formation of aerial rosettes at lateral meristems. Lines with overexpression of GLB1 or GLB2 bolted earlier than wild type plants. By germinating the lines in a medium containing the nitric oxide (NO) donor, sodium nitroprusside (SNP), it was demonstrated that both GLB1 and GLB2 promote bolting by antagonizing the effect of NO, suggesting that non-symbiotic plant hemoglobin controls bolting by scavenging the floral transition signal molecule, NO. So far, NO scavenging has only been demonstrated for class 1 non-symbiotic hemoglobins. A direct assay in Arabidopsis leaf cells shows that GLB1 as well as the class 2 non-symbiotic hemoglobin, GLB2, scavenge NO in vivo. NO has also been demonstrated to be a growth stimulating signal with an optimum at low concentrations. It was observed that overexpression of either GLB1 or GLB2 shifts the optimum for NO growth stimulation to a higher concentration. In conclusion, we have found that expression of NO scavenging plant hemoglobin is involved in the control of bolting in Arabidopsis.
Authors:
Kim Henrik Hebelstrup; Erik Ostergaard Jensen
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Publication Detail:
Type:  Journal Article     Date:  2007-11-22
Journal Detail:
Title:  Planta     Volume:  227     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-01-28     Completed Date:  2008-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  917-27     Citation Subset:  IM    
Affiliation:
Department of Molecular biology, Laboratory of Gene Expression, University of Aarhus, Gustav Wieds Vej 10C, 8000 Aarhus C, Denmark. khh@mb.au.dk
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics*,  growth & development,  metabolism
Arabidopsis Proteins / genetics*,  metabolism
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Plant / drug effects
Genotype
Hemoglobins / genetics*,  metabolism
Nitric Oxide / metabolism*
Nitroprusside / pharmacology
Phenotype
Plants, Genetically Modified
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Hemoglobins; 10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside

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