| Expression of NMDA receptor subunits in human peripheral blood lymphocytes in opioid addiction. | |
| | |
MedLine Citation:
|
PMID: 20420822 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Glutamate receptors especially the N-methyl-D-aspartate (NMDA)-activated ones have a key role in the development and maintenance of opioid addiction. It has been proposed that the neurotransmitter receptors expression in peripheral blood lymphocytes may be parallel to their expression state in the brain. This study was designed to evaluate the possibility of using the mRNA expression state of NR2A and NR3A subunits of NMDA receptors in human peripheral blood lymphocytes as a peripheral marker in opioid addiction studies. Four groups, each comprising of 20 male individuals participated in the study: opioid addicts, methadone maintained patients, long-term abstinent former opioid addicts, and non-addicted control subjects. Real-time PCR method was used to investigate the mRNA expression level of NR2A and NR3A subunits of NMDA receptors in peripheral blood lymphocytes of all groups. Our data indicated that the mRNA expression of NR2A subunit of NMDA receptors in all three test groups was not statistically different from control subjects. However, the NR3A subunit expression was significantly down-regulated in abstinent subjects reaching 0.14 the amount of the control group. The expression of NR3A subunit was not significantly changed in addicted and methadone maintained individuals in comparison to control subjects. It is concluded that the deficiency in expression of NR3A subunit of NMDA glutamate receptors detected by a peripheral marker may be a risk factor making individuals vulnerable for opioid addiction. |
| | |
Authors:
|
Reza Roozafzoon; Ali Goodarzi; Nasim Vousooghi; Mahmoud Sedaghati; Parichehr Yaghmaei; Mohammad-Reza Zarrindast |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-24 |
Journal Detail:
|
Title: European journal of pharmacology Volume: 638 ISSN: 1879-0712 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-04 Completed Date: 2010-09-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 29-32 Citation Subset: IM |
Copyright Information:
|
(c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
Science and Research Branch of Islamic Azad University, Tehran, Iran. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Markers
/
blood Down-Regulation* Humans Lymphocytes / metabolism* Male Opioid-Related Disorders / blood* RNA, Messenger / blood Receptors, N-Methyl-D-Aspartate / blood* |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/RNA, Messenger; 0/Receptors, N-Methyl-D-Aspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Plumbagin activates ERK1/2 and Akt via superoxide, Src and PI3-kinase in 3T3-L1 Cells.
Next Document: Maternal Embryonic Leucine zipper Kinase is stabilized in mitosis by phosphorylation and is partiall...