Document Detail


Expression of MyoD and myogenin in dystrophic mice, mdx and dy, during regeneration.
MedLine Citation:
PMID:  10867795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression of two myogenic regulatory factors, MyoD and myogenin, was studied in regenerating muscles of dystrophic mice and compared to a chemically induced regeneration process. First, the distribution of the two proteins was determined immunohistochemically at various time points after single administrations of a local anaesthetic, bupivacaine hydrochloride, which causes myonecrosis followed by regeneration. Detectable levels of MyoD appeared at 18 h and the expression reached their maximum levels at 48 h after the injection, which coincide with the stage when satellite cells are activated and start to proliferate. Myogenin became detectable in 24 h and its expression reached its highest level at 72 h after injection when newly formed myotubes appeared. The two genes were also expressed in the dystrophic muscles from dy and mdx mice which exhibit dystrophic pathological features but are associated with different phenotypes. In mdx mice the two genes were expressed at reasonably high levels in parallel with the active regenerating process, whereas in dy mice MyoD and myogenin expressions decreased as fibrosis progressed. However, MyoD was relatively more strongly expressed in the larger mature myotubes of dy mice than in those of mdx mice, suggesting prolonged regenerative activity. In dy and mdx mice, MyoD and myogenin were expressed in different quantities, indicating that these animals have distinct regenerating activities. Our findings confirm that expression of both MyoD and myogenin genes is necessary in the regenerative process for the proliferation of satellite cells (myoblasts) and for the development of early regenerating fibers (myotubes) even in dystrophic muscles.
Authors:
Y Jin; N Murakami; Y Saito; Y Goto; K Koishi; I Nonaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta neuropathologica     Volume:  99     ISSN:  0001-6322     ISO Abbreviation:  Acta Neuropathol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-09-28     Completed Date:  2000-09-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0412041     Medline TA:  Acta Neuropathol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  619-27     Citation Subset:  IM    
Affiliation:
Department of Ultrastructural Research, National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan. jin@ncnp.go.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Gene Expression Regulation, Developmental / physiology*
Mice
Mice, Inbred C57BL
Mice, Inbred mdx
Muscle Development
Muscle Fibers, Skeletal / metabolism,  pathology
Muscle, Skeletal / growth & development,  metabolism,  pathology
Muscular Dystrophies / metabolism*,  pathology,  physiopathology*
MyoD Protein / metabolism*
Myogenin / metabolism*
Rats
Rats, Wistar
Regeneration / genetics*
Chemical
Reg. No./Substance:
0/MyoD Protein; 0/Myog protein, mouse; 0/Myog protein, rat; 0/Myogenin

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