Document Detail

Expression of Myh9 in the mammalian cochlea: localization within the stereocilia.
MedLine Citation:
PMID:  16862555     Owner:  NLM     Status:  MEDLINE    
Mutations of non-muscle myosin Type IIA or MYH9 are linked to syndromic or nonsyndromic hearing loss. The biologic function of MYH9 in the auditory organ and the pathophysiology of its dysfunction remain to be determined. The mouse represents an excellent model for investigating the biologic role of MYH9 in the cells and tissues affected by its dysfunction. A primary step toward the understanding of the role of MYH9 in hearing and its dysfunction is the documentation of its cellular and sub-cellular localization within the cochlea, the auditory organ. We describe the localization of Myh9 within the mouse cochlea using a polyclonal anti-Myh9-antibody, generated against an 18 amino acid long peptide corresponding to the sequence at the C-terminus of mouse Myh9. The anti-Myh9 antibody identified a single, specific, immunoreactive band of 220 kDa in immunoblot analysis of homogenate from a variety of different mouse tissues. The Myh9 antibody cross-reacts with the rat but not the human orthologue. Myh9 is expressed predominantly within the spiral ligament as well as in the sensory hair cells of the organ of Corti. Confocal microscopy of cochlear surface preparations, identified Myh9 within the inner and outer hair cells and their stereocilia. Localization of Myh9 within the stereocilia raises the possibility that mutations of MYH9 may effect hearing loss though disruption of the stereocilia structure.
Anand N Mhatre; Yan Li; Graham Atkin; Abdel Maghnouj; Anil K Lalwani
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  84     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-30     Completed Date:  2006-10-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  809-18     Citation Subset:  IM    
Laboratory of Molecular Otology, Department of Otolaryngology, New York University School of Medicine, New York, New York 10016, USA.
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MeSH Terms
Blotting, Western / methods
Cilia / metabolism*
Cochlea / cytology*
Gene Expression / physiology*
Immunohistochemistry / methods
Nonmuscle Myosin Type IIA / genetics,  metabolism*
Grant Support
Reg. No./Substance:
EC 3.6.1.-/Nonmuscle Myosin Type IIA

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