| Expression of MicroRNA-146a in osteoarthritis cartilage. | |
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MedLine Citation:
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PMID: 19333945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: A role of microRNA, which are approximately 22-nucleotide noncoding RNAs, has recently been recognized in human diseases. The objective of this study was to identify the expression pattern of microRNA-146a (miR-146a) in cartilage from patients with osteoarthritis (OA). METHODS: The expression of miR-146a in cartilage from 15 patients with OA was analyzed by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and by in situ hybridization. Induction of the expression of miR-146a by cultures of normal human articular chondrocytes following stimulation with interleukin-1beta (IL-1beta) was examined by quantitative RT-PCR. RESULTS: All cartilage samples were divided into 3 groups according to a modification of the Mankin score (grade I = mild OA scored 0-5, grade II = moderate OA scored 6-10, and grade III = severe OA scored 11-14). In grade I OA cartilage samples, the expression of miR-146a and COL2A1 was significantly higher than that in the other groups (P < 0.05). In grades II and III OA cartilage, the expression of miR-146a and COL2A1 was decreased, whereas the expression of matrix metalloproteinase 13 (MMP-13) was elevated in grade II OA cartilage. These data showed that miR-146a is expressed intensely in cartilage with a low Mankin grade and that miR-146a expression decreases in parallel with the level of MMP-13 expression. Tissue section in situ hybridization of primary miR-146a (pri-miR-146a) revealed that pri-miR-146a was expressed in chondrocytes residing in all tissue layers, especially in the superficial layer, where it was intensely expressed. The expression of miR-146 was markedly elevated by IL-1beta stimulation in human chondrocytes in vitro. CONCLUSION: This study shows that miR-146 is intensely expressed in low-grade OA cartilage and that its expression is induced by stimulation of IL-1beta. Thus, miR-146 might play a role in OA cartilage pathogenesis. |
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Authors:
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Keiichiro Yamasaki; Tomoyuki Nakasa; Shigeru Miyaki; Masakazu Ishikawa; Masataka Deie; Nobuo Adachi; Yuji Yasunaga; Hiroshi Asahara; Mitsuo Ochi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Arthritis and rheumatism Volume: 60 ISSN: 0004-3591 ISO Abbreviation: Arthritis Rheum. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-07 Completed Date: 2009-06-22 Revised Date: 2013-06-02 |
Medline Journal Info:
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Nlm Unique ID: 0370605 Medline TA: Arthritis Rheum Country: United States |
Other Details:
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Languages: eng Pagination: 1035-41 Citation Subset: AIM; IM |
Affiliation:
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Hiroshima University, Hiroshima, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Aged Cartilage, Articular / cytology, physiology* Cells, Cultured Chondrocytes / cytology, drug effects, physiology Collagen Type II / genetics Female Gene Expression Regulation / physiology Humans Interleukin-1beta / pharmacology Male Matrix Metalloproteinase 13 / genetics MicroRNAs / genetics* Middle Aged Osteoarthritis / genetics*, pathology, physiopathology* Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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AR-50631/AR/NIAMS NIH HHS; AR-56120/AR/NIAMS NIH HHS; R01 AR050631-05/AR/NIAMS NIH HHS; R21 AR056120-01A2/AR/NIAMS NIH HHS; R56 AR050631-06/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/COL2A1 protein, human; 0/Collagen Type II; 0/Interleukin-1beta; 0/MIRN146 microRNA, human; 0/MicroRNAs; EC 3.4.24.-/Matrix Metalloproteinase 13 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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