Document Detail


Expression of MAL, an integral protein component of the machinery for raft-mediated pical transport, in human epithelia.
MedLine Citation:
PMID:  12704214     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The MAL protein is the only integral membrane protein identified as being an essential component of the machinery necessary for apical transport in the canine MDCK cell line, a paradigm of polarized epithelial cells. To characterize the range of human epithelia that use MAL-mediated pathways of transport, we performed an immunohistochemical survey of normal tissues using a monoclonal antibody (MAb) specific for the MAL protein. For comparison, different types of carcinoma were also analyzed. MAL, with a characteristic strong supranuclear granular distribution, was detected in specific types of normal epithelial cells throughout the respiratory system, the gastrointestinal and genitourinary tracts, and in exocrine and endocrine glands. Absorptive cells (e.g., enterocytes), and many different types of specialized secretory cells, either organized in discrete clusters (e.g., endocrine cells in the pancreas), gathered together in an endocrine gland (e.g., thyroid), interspersed with other cells in glands (e.g., parietal cells), or dispersed singly among other cells (e.g., type 2 pneumocytes) were positive for MAL. We also analyzed a series of epithelial renal and thyroid tumors and found alterations dependent on the particular histological type of tumor. These results open potential applications of the anti-MAL antibody for the characterization of neoplastic tissue.
Authors:
Mónica Marazuela; Agustín Acevedo; Magdalena Adrados; María Angeles García-López; Miguel A Alonso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  51     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-21     Completed Date:  2003-05-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  665-74     Citation Subset:  IM    
Affiliation:
Departmento de Endocrinología, Cantoblanco, Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Carcinoma / metabolism
Epithelium / metabolism*
Humans
Immunohistochemistry
Kidney Neoplasms / metabolism
Membrane Microdomains / physiology*
Membrane Transport Proteins*
Myelin Proteins*
Organ Specificity
Proteolipids / metabolism*
Thyroid Neoplasms / metabolism
Chemical
Reg. No./Substance:
0/MAL protein, T-cell; 0/Membrane Transport Proteins; 0/Myelin Proteins; 0/Proteolipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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