Document Detail


Expression of lymphatic markers during avian and mouse cardiogenesis.
MedLine Citation:
PMID:  19938109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The adult heart has been reported to have an extensive lymphatic system, yet the development of this important system during cardiogenesis is still largely unexplored. The nuclear-localized transcription factor Prox-1 identified a sheet of Prox-1-positive cells on the developing aorta and pulmonary trunk in avian and murine embryos just before septation of the four heart chambers. The cells coalesced into a branching lymphatic network that spread within the epicardium to cover the heart. These vessels eventually expressed the lymphatic markers LYVE-1, VEGFR-3, and podoplanin. Before the Prox-1-positive cells were detected in the mouse epicardium, LYVE-1, a homologue of the CD44 glycoprotein, was primarily expressed in individual epicardial cells. Similar staining patterns were observed for CD44 in avian embryos. The proximity of these LYVE-1/CD44-positive mesenchymal cells to Prox-1-positive vessels suggests that they may become incorporated into the lymphatics. Unexpectedly, we detected LYVE-1/PECAM/VEGFR-3-positive vessels within the embryonic and adult myocardium, which remained Prox-1/podoplanin-negative. Lymphatic markers were surprisingly found in adult rat and embryonic mouse epicardial cell lines, with Prox-1 also exhibiting nuclear-localized expression in primary cultures of embryonic avian epicardial cells. Our data identified three types of cells in the embryonic heart expressing lymphatic markers: (1) Prox-1-positive cells from an extracardiac source that migrate within the serosa of the outflow tract into the epicardium of the developing heart, (2) individual LYVE-1-positive cells in the epicardium that may be incorporated into the Prox-1-positive lymphatic vasculature, and (3) LYVE-1-positive cells/vessels in the myocardium that do not become Prox-1-positive even in the adult heart.
Authors:
Ganga Karunamuni; Ke Yang; Yong Qiu Doughman; Jamie Wikenheiser; David Bader; Joey Barnett; Anita Austin; Patricia Parsons-Wingerter; Michiko Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Anatomical record (Hoboken, N.J. : 2007)     Volume:  293     ISSN:  1932-8494     ISO Abbreviation:  Anat Rec (Hoboken)     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-01     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  101292775     Medline TA:  Anat Rec (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-70     Citation Subset:  IM    
Copyright Information:
2009 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD31 / metabolism
Antigens, CD44
Avian Proteins / metabolism
Biological Markers / metabolism*
Blotting, Western
Cells, Cultured
Chick Embryo
Chickens
Gestational Age
Glycoproteins / metabolism
Heart / embryology*
Homeodomain Proteins / metabolism
Immunohistochemistry
Lymphatic System / embryology*,  metabolism*
Membrane Glycoproteins / metabolism
Mice
Morphogenesis
Myocardium / metabolism*
Pericardium / embryology,  metabolism
Quail
Rats
Tumor Suppressor Proteins / metabolism
Vascular Endothelial Growth Factor Receptor-3 / metabolism
Grant Support
ID/Acronym/Agency:
HL0775436/HL/NHLBI NIH HHS; HL083048/HL/NHLBI NIH HHS; HL085708/HL/NHLBI NIH HHS; HL65314/HL/NHLBI NIH HHS; R01 ES013507/ES/NIEHS NIH HHS; R01 HL091171/HL/NHLBI NIH HHS; R01 HL091171-01A1/HL/NHLBI NIH HHS; R01 HL091171-02/HL/NHLBI NIH HHS; R21 ES013507/ES/NIEHS NIH HHS; R21 ES013507-01/ES/NIEHS NIH HHS; R21 ES013507-02/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD31; 0/Antigens, CD44; 0/Avian Proteins; 0/Biological Markers; 0/Cd44 protein, mouse; 0/Glycoproteins; 0/Gp38 protein, mouse; 0/Homeodomain Proteins; 0/Membrane Glycoproteins; 0/Tumor Suppressor Proteins; 0/Xlkd1 protein, mouse; 0/prospero-related homeobox 1 protein; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-3
Comments/Corrections

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