| Expression of lymphatic markers during avian and mouse cardiogenesis. | |
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MedLine Citation:
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PMID: 19938109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The adult heart has been reported to have an extensive lymphatic system, yet the development of this important system during cardiogenesis is still largely unexplored. The nuclear-localized transcription factor Prox-1 identified a sheet of Prox-1-positive cells on the developing aorta and pulmonary trunk in avian and murine embryos just before septation of the four heart chambers. The cells coalesced into a branching lymphatic network that spread within the epicardium to cover the heart. These vessels eventually expressed the lymphatic markers LYVE-1, VEGFR-3, and podoplanin. Before the Prox-1-positive cells were detected in the mouse epicardium, LYVE-1, a homologue of the CD44 glycoprotein, was primarily expressed in individual epicardial cells. Similar staining patterns were observed for CD44 in avian embryos. The proximity of these LYVE-1/CD44-positive mesenchymal cells to Prox-1-positive vessels suggests that they may become incorporated into the lymphatics. Unexpectedly, we detected LYVE-1/PECAM/VEGFR-3-positive vessels within the embryonic and adult myocardium, which remained Prox-1/podoplanin-negative. Lymphatic markers were surprisingly found in adult rat and embryonic mouse epicardial cell lines, with Prox-1 also exhibiting nuclear-localized expression in primary cultures of embryonic avian epicardial cells. Our data identified three types of cells in the embryonic heart expressing lymphatic markers: (1) Prox-1-positive cells from an extracardiac source that migrate within the serosa of the outflow tract into the epicardium of the developing heart, (2) individual LYVE-1-positive cells in the epicardium that may be incorporated into the Prox-1-positive lymphatic vasculature, and (3) LYVE-1-positive cells/vessels in the myocardium that do not become Prox-1-positive even in the adult heart. |
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Authors:
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Ganga Karunamuni; Ke Yang; Yong Qiu Doughman; Jamie Wikenheiser; David Bader; Joey Barnett; Anita Austin; Patricia Parsons-Wingerter; Michiko Watanabe |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Anatomical record (Hoboken, N.J. : 2007) Volume: 293 ISSN: 1932-8494 ISO Abbreviation: Anat Rec (Hoboken) Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-01 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 101292775 Medline TA: Anat Rec (Hoboken) Country: United States |
Other Details:
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Languages: eng Pagination: 259-70 Citation Subset: IM |
Copyright Information:
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2009 Wiley-Liss, Inc. |
Affiliation:
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Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD31 / metabolism Antigens, CD44 Avian Proteins / metabolism Biological Markers / metabolism* Blotting, Western Cells, Cultured Chick Embryo Chickens Gestational Age Glycoproteins / metabolism Heart / embryology* Homeodomain Proteins / metabolism Immunohistochemistry Lymphatic System / embryology*, metabolism* Membrane Glycoproteins / metabolism Mice Morphogenesis Myocardium / metabolism* Pericardium / embryology, metabolism Quail Rats Tumor Suppressor Proteins / metabolism Vascular Endothelial Growth Factor Receptor-3 / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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HL0775436/HL/NHLBI NIH HHS; HL083048/HL/NHLBI NIH HHS; HL085708/HL/NHLBI NIH HHS; HL65314/HL/NHLBI NIH HHS; R01 ES013507/ES/NIEHS NIH HHS; R01 HL091171-01A1/HL/NHLBI NIH HHS; R01 HL091171-02/HL/NHLBI NIH HHS; R21 ES013507-01/ES/NIEHS NIH HHS; R21 ES013507-02/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD31; 0/Antigens, CD44; 0/Avian Proteins; 0/Biological Markers; 0/Cd44 protein, mouse; 0/Glycoproteins; 0/Gp38 protein, mouse; 0/Homeodomain Proteins; 0/Membrane Glycoproteins; 0/Tumor Suppressor Proteins; 0/Xlkd1 protein, mouse; 0/prospero-related homeobox 1 protein; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-3 |
| Comments/Corrections | |
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