Document Detail


Expression of Lymphangiogenic Vascular Endothelial Growth Factor Family Members in Bovine Corpus Luteum.
MedLine Citation:
PMID:  23126445     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of this study was to evaluate mRNA expression, protein concentration and localization of the assumedly important lymphangiogenic factors VEGFC and VEGFD and the receptor FLT4 in bovine corpora lutea (CL) during different physiological stages. In experiment 1, CL were collected in a slaughterhouse and stages (days 1-2, 3-4, 5-7, 8-12, 13-16, >18) of oestrous cycle and month <3, 3-5, 6-7 and >8 of pregnancy. In experiment 2, prostaglandin F2α (PGF)-induced luteolysis was performed in 30 cows, which were injected with PGF analogue on day 8-12 (mid-luteal phase), and CL were collected before and 0.5, 2, 4, 12, 24, 48 and 64 h after PGF injection. The mRNA expression was characterized by RT-qPCR. All three factors were clearly expressed and showed significant changes during different groups and periods examined in both experiments. Protein concentrations of VEGFD and FLT4 measured by ELISA were not detectable in early cyclic CL but increased to higher plateau levels during pregnancy. After PGF-induced luteolysis FLT4 protein showed an increase within 2-24 h after the injection. FLT4 localization by immunohistochemistry in the cytoplasm of luteal cells was relatively weak in early CL. It increased in late CL and especially in CL during pregnancy. During pregnancy, a positive FLT4 staining in both the nucleus and cytoplasm of lymphatic endothelial cells in peripheral tissue was observed. In conclusion, our results lead to the assumption that lymphangiogenic factors are produced and regulated in CL and may be involved in mechanisms regulating CL function, especially during pregnancy.
Authors:
B Berisha; S Schilffarth; R Kenngott; F Sinowatz; H H D Meyer; D Schams
Related Documents :
24265885 - A comparative study of vascular cell adhesion molecule-1 and high-sensitive c-reactive ...
18342435 - An autopsy case of acute multiple sclerosis (marburg's type) during pregnancy.
22115165 - Pregnancy and other pituitary disorders (including gh deficiency).
11229185 - Cerebral venous thrombosis in pregnancy and puerperium--a prospective study.
8582515 - Prediction of early pregnancy outcomes.
23555035 - Predictive factors of plasma hiv suppression during pregnancy: a prospective cohort stu...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-5
Journal Detail:
Title:  Anatomia, histologia, embryologia     Volume:  -     ISSN:  1439-0264     ISO Abbreviation:  Anat Histol Embryol     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7704218     Medline TA:  Anat Histol Embryol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Verlag GmbH.
Affiliation:
Physiology Weihenstephan, Technische Universität München, Weihenstephaner Berg 3, 85354, Freising, Germany; Faculty of Agriculture and Veterinary, University of Prishtina, Bulevardi Bill Clinton p.n, 10000, Prishtinë, Kosovo.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Useful and convenient procedure for intermittent vascular occlusion in laparoscopic hepatectomy.
Next Document:  Rhodium-Catalyzed Carbon-Silicon Bond Activation for Synthesis of Benzosilole Derivatives.