| Expression of Ki-67 in the uterus during various stages of the estrous cycle in rats. | |
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MedLine Citation:
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PMID: 17855063 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rats have an average estrous cycle of 4-5 days. There are four phases (proestrus, estrus, metesterus, and diestrus) in the estrous cycle in rodents. Histologic staging of the rodent estrous cycle is challenging and requires expertise. Thus, utilizing additional parameters such as cellular proliferation of the various components of the uterine microanatomy may assist with this process. Having an alternative method by which a pathologist can correctly identify the stages of the rodent estrous cycle would be valuable to the assessment and interpretation of safety studies for new drug candidates. This study was performed to investigate the microanatomic location of the uterine proliferative activity by image analysis and immunohistochemistry using Ki-67, a well-established marker of proliferating cells. Each stage of the rodent estrous cycle exhibited a different pattern of cellular proliferation. During proestrus, the lowest degree of cellular proliferation occurred in the glandular epithelial cells and the highest occurred in the myometrial cells. In estrus, lower levels of cellular proliferation were seen in the luminal and glandular epithelial cells, while a higher rate of proliferation occurred in myometrial cells followed by the stromal cells. At the metestrus stage, the highest cellular proliferation occurred in stromal and myometrial cells, while lesser proliferation was observed in luminal and glandular epithelial cells. This work demonstrates that in the rodent uterus there are cyclic changes in cellular proliferation in specific microanatomic uterine locations which can aid in the staging of the estrous cycle. |
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Authors:
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Rosemary A Marusak; Zaher A Radi; Leslie Obert |
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Publication Detail:
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Type: Journal Article Date: 2007-09-12 |
Journal Detail:
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Title: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie Volume: 59 ISSN: 0940-2993 ISO Abbreviation: Exp. Toxicol. Pathol. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-09 Completed Date: 2008-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9208920 Medline TA: Exp Toxicol Pathol Country: Germany |
Other Details:
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Languages: eng Pagination: 151-5 Citation Subset: IM |
Affiliation:
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Michigan State University, East Lansing, MI, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / metabolism Cell Count Cell Proliferation Estrous Cycle / metabolism* Female Fluorescent Antibody Technique, Indirect Image Processing, Computer-Assisted Immunoenzyme Techniques Ki-67 Antigen / metabolism* Rats Uterus / anatomy & histology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Ki-67 Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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