Document Detail


Expression of insulin-like growth factor (IGF) family members in porcine pregnancy.
MedLine Citation:
PMID:  21685713     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prenatal mortality is a prime concern for commercial swine industry in North America. Fetal losses occur throughout gestation but cluster in early (~day20) and mid (~day50) pregnancy. Adequate vascularization of the attachment site has emerged as a key factor contributing to fetal success. Since Insulin-Like Growth Factor (IGF) family members regulate angiogenesis in addition to promoting fetal development and growth, we hypothesized that conceptus success is governed by members of the IGF family. Using quantitative real time PCR, we analyzed expression of IGF family members (IGF-I, IGF-II, IGF-I Receptor (IGF-IR), IGF-IIR and their binding proteins, IGFBPs) in matched maternal and fetal tissues of healthy and arresting conceptuses at gestation days (gd) 20 and 50. IGF-II transcripts were 100 fold increased in both maternal and fetal tissues compared to IGF-I, but receptor transcripts were found in similar abundance irrespective of health status and gestation point. IGFBP3 was the most abundantly transcribed of the binding proteins. Using immunohistochemistry we confirmed the expression of IGF family members in maternal luminal and glandular epithelial cells, the endothelium of blood vessels and some scattered stromal cells. Our results suggest that IGF-I and II and their receptors are differentially expressed at the maternal and fetal components of the attachment site.
Authors:
Germaine Miese-Looy; Marianne J VAN DEN Heuvel; Andrew K Edwards; Jonathan Lamarre; Chandrakant Tayade
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-17
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  58     ISSN:  1348-4400     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-27     Completed Date:  2012-07-31     Revised Date:  2012-08-24    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  51-60     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryo, Mammalian / blood supply,  cytology,  metabolism*
Female
Immunohistochemistry
Insulin-Like Growth Factor Binding Proteins / biosynthesis*
Insulin-Like Growth Factor I / biosynthesis*
Insulin-Like Growth Factor II / biosynthesis*
Pregnancy
Receptor, IGF Type 1 / biosynthesis*
Receptor, IGF Type 2 / biosynthesis*
Swine
Chemical
Reg. No./Substance:
0/Insulin-Like Growth Factor Binding Proteins; 0/Receptor, IGF Type 2; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II; EC 2.7.10.1/Receptor, IGF Type 1

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