Document Detail


Expression of Hsp70 in the mud crab, Scylla paramamosain in response to bacterial, osmotic, and thermal stress.
MedLine Citation:
PMID:  23325574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hsp70 is involved in immune responses against infectious pathogens, thermal, and osmotic stress. To understand the immune defense mechanisms of the mud crab Scylla paramamosain, genomic DNA, transcript level and antimicrobial activities of Hsp70 were analyzed. Genomic DNA sequence analysis revealed one intron in this gene. Furthermore, six SNPs were detected by direct sequencing from 30 samples in this study. Hsp70 mRNA was expressed in almost all tissues examined. By using the quantitative real-time PCR, the expression level of Hsp70 in hemocytes showed a clear time-dependent expression pattern during the 96 h after stimulated by Vibrio alginolyticus. Then, recombinant Hsp70 was obtained by using the bacterial expression system, but no obvious antimicrobial activity has been found for the protein in the antimicrobial tests. After osmotic stress, the expression of Hsp70 in hemocytes showed this gene was induced by the high salinity (30 ‰) for at least 96 h. Hsp70 mRNA expression in hemocytes was analyzed after thermal stress at 6 h, the highest and the lowest expression level of Hsp70 was observed at 36 and 15 °C, respectively. These results indicated that Hsp70 was inducible by bacterial, osmotic, and thermal stress, and therefore plays an important role, different from antibacterial peptide, in innate immune responses of S. paramamosain.
Authors:
Ya'nan Yang; Haihui Ye; Huiyang Huang; Shaojing Li; Xueliang Liu; Xianglan Zeng; Jie Gong
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Publication Detail:
Type:  Journal Article     Date:  2013-01-17
Journal Detail:
Title:  Cell stress & chaperones     Volume:  18     ISSN:  1466-1268     ISO Abbreviation:  Cell Stress Chaperones     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-14     Completed Date:  2013-12-30     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  9610925     Medline TA:  Cell Stress Chaperones     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  475-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brachyura / genetics,  metabolism*
Genome
HSP70 Heat-Shock Proteins / genetics,  metabolism*
Hemocytes / metabolism,  microbiology
Mutation
Osmotic Pressure
RNA, Messenger / metabolism
Recombinant Proteins / biosynthesis,  genetics,  pharmacology
Stress, Physiological*
Time Factors
Vibrio alginolyticus / drug effects,  physiology
Chemical
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/RNA, Messenger; 0/Recombinant Proteins
Comments/Corrections

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