Document Detail

Expression of Glial Cell Line-Derived Neurotrophic Factor and its Receptors in Cultured Retinal Müller Cells Under High Glucose Circumstance.
MedLine Citation:
PMID:  22266674     Owner:  NLM     Status:  Publisher    
This study aimed to explore the effect of high glucose concentration on the expression of glial cell line-derived neurotrophic factor (GDNF) and its family ligand receptors (GFRs) GFRα1 and GFRα2 in Müller cells and the protective role of GDNF in cultured Müller cells under high glucose circumstance. Cultured Müller cells (untreated or treated with 200 ng/mL of GDNF) were exposed to high glucose conditions (20 mmol/L glucose). We found that the expression levels of GDNF and GFRα1 mRNA and protein increased gradually over time under high glucose and exogenous GDNF-treated conditions, whereas the upregulation in GFRα2 expression was observed only in the early stage of high glucose conditions. Exogenous GDNF not only decreased apoptosis in cultured Müller cells under high glucose circumstance, but also accelerated the levels and speed of synthesis of GDNF and GFRα1 proteins in Müller cells. These results suggest that Müller cells can synthesize GDNF and GFRs under high glucose conditions, and GDNF may play important role in protecting Müller cells during the early stage of diabetic retinopathy. The difference in GFRs expression indicated that GDNF and neurturin may exert different effects on Müller cells under high glucose circumstance. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.
Xinping Zhu; Yan Sun; Zhongping Wang; Weigang Cui; Yuwen Peng; Ruixi Li
Related Documents :
11438734 - Guanylyl cyclase c agonists regulate progression through the cell cycle of human colon ...
9405104 - Anteroposterior gradient of epithelial transformation during amphibian intestinal remod...
16809224 - Interstitial cells of cajal in patients with constipation due to total colonic inertia.
1175214 - Lymphoid cells in chicken intestinal epithelium.
10673504 - Absence of wee1 ensures the meiotic cell cycle in xenopus oocytes.
22584374 - The reciprocal relationship between primordial germ cells and pluripotent stem cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-20
Journal Detail:
Title:  Anatomical record (Hoboken, N.J. : 2007)     Volume:  -     ISSN:  1932-8494     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101292775     Medline TA:  Anat Rec (Hoboken)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Department of Anatomy, Histology and Embryology, Shanghai Medical College, Fudan University, Shanghai, China; Department of Ultrasound, East Hospital, Tongji University, Shanghai, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Contemporary Epidemiology of North American Lineage Triple Reassortant Influenza A Viruses in Pigs.
Next Document:  Layered hybrid organic-inorganic nanobelts: synthesis and removal of trace heavy metal ions.