| Expression of genes associated with aroma formation derived from the fatty acid pathway during peach fruit ripening. | |
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MedLine Citation:
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PMID: 20415420 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Changes in characteristic aroma volatiles, levels of fatty acids as aroma precursors, and expression patterns of related genes, including lipoxygenase (LOX), hydroperoxide lyase (HPL), alcohol dehydrogenase (ADH), alcohol acyltransferase (AAT), and fatty acid desaturase (FAD), were studied in peach ( Prunus persica L. Batsch., cv. Yulu) fruit during postharvest ripening at 20 degrees C. Concentrations of n-hexanal, (E)-2-hexenal, (E)-2-hexenol, and (Z)-3-hexenol decreased, whereas the production of (Z)-3-hexenyl acetate, gamma-hexalactone, gamma-octalactone, gamma-decalactone, and delta-decalactone increased with fruit ripening. Lactones showed a clear pattern concomitant with the climacteric rise in ethylene production, with gamma-decalactone being the principal volatile compound at the late ripening stage. Of the LOX family genes, PpLOX2 and PpLOX3 had relatively high transcript levels initially followed by a decline with fruit ripening, while levels of PpLOX1 and PpLOX4 transcripts were upregulated by accumulated ethylene production. Expression of PpHPL1, PpADH1, PpADH2, and PpADH3 showed similar decreasing patterns during ripening. Expression levels of PpAAT1 showed a rapid increase during the first 2 days of postharvest ripening followed by a gradual decrease. Contents of polyunsaturated linoleic and linolenic acids increased, and saturated palmitic acid levels tended to decline as the fruit ripened. The increased levels of unsaturated fatty acids closely paralleled increasing expression of PpFAD1 and PpFAD2. The significance of gene expression changes in relation to aroma volatile production is discussed. |
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Authors:
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Bo Zhang; Ji-Yuan Shen; Wen-Wen Wei; Wan-Peng Xi; Chang-Jie Xu; Ian Ferguson; Kunsong Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-20 Completed Date: 2010-10-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 6157-65 Citation Subset: IM |
Affiliation:
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Laboratory of Fruit Quality Biology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acyltransferases
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genetics Alcohol Dehydrogenase / genetics Aldehyde-Lyases / genetics Cytochrome P-450 Enzyme System / genetics Ethylenes / biosynthesis Fatty Acid Desaturases / genetics Fatty Acids / analysis, metabolism* Fruit / chemistry, genetics*, growth & development* Gene Expression* Lactones / analysis Lipoxygenase / genetics Odors / analysis* Prunus / genetics* Volatile Organic Compounds / analysis, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Ethylenes; 0/Fatty Acids; 0/Lactones; 0/Volatile Organic Compounds; 74-85-1/ethylene; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.1.1.1/Alcohol Dehydrogenase; EC 1.13.11.12/Lipoxygenase; EC 1.14.19.-/Fatty Acid Desaturases; EC 2.3.-/Acyltransferases; EC 4.1.2.-/Aldehyde-Lyases; EC 4.1.2.-/hydroperoxide lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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