Document Detail

Expression of GABA(A) receptor alpha3-, theta-, and epsilon-subunit mRNAs during rat CNS development and immunolocalization of the epsilon subunit in developing postnatal spinal cord.
MedLine Citation:
PMID:  19249336     Owner:  NLM     Status:  MEDLINE    
Ionotropic GABA(A) receptors are heteromeric structures composed of a combination of five from at least 16 different subunits. Subunit genes are expressed in distinct cell types at specific times during development. The most abundant native GABA(A) receptors consist of alpha1-, beta2-, and gamma2-subunits that are co-expressed in numerous brain areas. alpha3-, theta-, And epsilon-subunits are clustered on the X chromosome and show striking overlapping expression patterns throughout the adult rat brain. To establish whether these subunits are temporally and spatially co-expressed, we used in situ hybridization to analyze their expression throughout rat development from embryonic stage E14 to postnatal stage P12. Each transcript exhibited a unique or a shared regional and temporal developmental expression profile. The thalamic expression pattern evolved from a restricted expression of epsilon and theta transcripts before birth, to a theta and alpha3 expression at birth, and finally to a grouped epsilon, theta and alpha3 expression postpartum. However, strong similarities occurred, such as a grouped expression of the three subunits within the hypothalamus, tegmentum and pontine nuclei throughout the developmental process. At early stages of development (E17), epsilon and theta appeared to have a greater spatial distribution before the dominance of the alpha3 subunit transcript around birth. We also revealed expression of alpha3, theta, and epsilon in the developing spinal cord and identified neurons that express epsilon in the postnatal dorsal horn, intermediolateral column and motoneurons. Our findings suggest that various combinations of alpha3-, theta- and epsilon-subunits may be assembled at a regional and developmental level in the brain.
J-R Pape; S S Bertrand; P Lafon; M-F Odessa; M Chaigniau; J K Stiles; M Garret
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-26
Journal Detail:
Title:  Neuroscience     Volume:  160     ISSN:  1873-7544     ISO Abbreviation:  Neuroscience     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-13     Completed Date:  2009-08-17     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  85-96     Citation Subset:  IM    
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MeSH Terms
Blotting, Western
Brain / embryology*,  growth & development*
In Situ Hybridization
Neurons / metabolism
Protein Subunits / metabolism
RNA, Messenger / metabolism
Rats, Wistar
Receptors, GABA-A / metabolism*
Spinal Cord / embryology*,  growth & development*
Grant Support
S06 GM008248/GM/NIGMS NIH HHS; S06 GM008248-160052/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Gabra3 protein, rat; 0/Protein Subunits; 0/RNA, Messenger; 0/Receptors, GABA-A

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