| Expression of G1 cell cycle markers and the effect of adenovirus-mediated overexpression of p21Waf-1 in squamous cell carcinoma of the esophagus. | |
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MedLine Citation:
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PMID: 11115554 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine the crucial abnormality in the cell cycle regulatory proteins in human squamous cell carcinoma of the esophagus, we examined the cell growth ratio (CGR) and basal expression levels of G1 cyclins (cyclin D1, cyclin E), cyclin-dependent kinase (cdk) 2, cdk4, proliferating cell nuclear antigen (PCNA), and p21Waf-1 using 9 cell lines (KE3, KE4, TE8, TE9, TE10, TE11, YES1, YES2, and YES6). Western blotting revealed an inverse linear correlation between the basal levels of p21Waf-1 expression and CGR. The protein levels of G1 cyclins, cdks, and PCNA did not coordinately reflect the CGR. There was no relationship between p21Waf-1 expression levels and mutation of the p53 gene. Next, when the cells were stimulated with serum 48 h after the starvation, stimulated levels of the above G1 cell cycle markers were variously observed among cell lines irrespective of CGR. Serum stimulation markedly induced phosphorylated Rb in TE9 (a high CGR cell line, CGR>2.0), but not in KE4 (a low CGR cell line, CGR<1.5). Furthermore, adenovirus-mediated expression of exogenous p21Waf-1 effectively reduced cell growth in KE3 and TE9 (high CGR cell lines), but not in KE4 and TE11 (low CGR cell lines). p21Waf-1-mediated growth suppression was associated with the induction of involucrin, a marker of squamous cell differentiation. Our data suggested that the basal level, but not the stimulated level, of p21Waf-1 expression play a pivotal role in abnormal growth in human squamous cell carcinoma of the esophagus. |
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Authors:
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T Fujii; S Kato; H Yamana; Y Tanaka; H Fujita; K Shirouzu; M Morimatsu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of oncology Volume: 18 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-08 Completed Date: 2001-02-15 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: GREECE |
Other Details:
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Languages: eng Pagination: 157-63 Citation Subset: IM |
Affiliation:
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Department of Pathology, Kurume University, School of Medicine, Kurume 830-0011, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics Carcinoma, Squamous Cell / pathology* Cell Differentiation / drug effects Cell Division / drug effects Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinases / biosynthesis Cyclins / biosynthesis, genetics, pharmacology* Esophageal Neoplasms / pathology* G1 Phase / physiology* Gene Transfer Techniques Genetic Vectors Humans Transgenes / physiology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; EC 2.7.11.22/Cyclin-Dependent Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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