Document Detail

Expression and function of fibroblast growth factor 18 in the ovarian follicle in cattle.
MedLine Citation:
PMID:  20484739     Owner:  NLM     Status:  MEDLINE    
Fibroblast growth factors (FGF) are involved in paracrine signaling between cell types in the ovarian follicle. FGF8, for example, is secreted by oocytes and controls cumulus cell metabolism. The closely related FGF18 is also expressed in oocytes in mice. The objective of this study was to assess the potential role of FGF18 in follicle growth in a monovulatory species, the cow. Messenger RNA encoding FGF18 was detected primarily in theca cells, and in contrast to the mouse, FGF18 was not detected in bovine oocytes. Addition of FGF18 protein to granulosa cell cultures inhibited estradiol and progesterone secretion as well as the abundance of mRNA encoding steroidogenic enzymes and the follicle-stimulating hormone receptor. In vivo, onset of atresia of the subordinate follicle was associated with increased thecal FGF18 mRNA levels and FGF18 protein in follicular fluid. In vitro, FGF18 altered cell cycle progression as measured by flow cytometry, resulting in increased numbers of dead cells (sub-G1 peak) and decreased cells in S phase. This was accompanied by decreased levels of mRNA encoding the cell cycle checkpoint regulator GADD45B. Collectively, these data point to a unique role for this FGF in signaling from theca cells to granulosa cells and suggest that FGF18 influences the process of atresia in ovarian follicles.
Valerio M Portela; Mariana Machado; Jose Buratini; Gustavo Zamberlam; Renee L Amorim; Paulo Goncalves; Christopher A Price
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-19
Journal Detail:
Title:  Biology of reproduction     Volume:  83     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  339-46     Citation Subset:  IM    
Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Quebec, Canada.
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MeSH Terms
Antigens, Differentiation / metabolism
Blotting, Western
Cell Cycle / physiology
Cells, Cultured
Estradiol / secretion
Fibroblast Growth Factors / physiology*
Flow Cytometry
Follicular Atresia / physiology
Granulosa Cells / metabolism*,  secretion
Ovarian Follicle / cytology,  physiology*
Paracrine Communication / physiology*
Progesterone / secretion
RNA, Messenger / metabolism
Receptors, FSH / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Theca Cells / metabolism*
Tissue Culture Techniques
Reg. No./Substance:
0/Antigens, Differentiation; 0/RNA, Messenger; 0/Receptors, FSH; 0/fibroblast growth factor 18; 50-28-2/Estradiol; 57-83-0/Progesterone; 62031-54-3/Fibroblast Growth Factors
Comment In:
Biol Reprod. 2010 Sep;83(3):322-4   [PMID:  20610810 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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