| Expression and function of apoptosis initiator FOXO3 in granulosa cells during follicular atresia in pig ovaries. | |
| | |
MedLine Citation:
|
PMID: 21071886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In mammalian ovaries, most follicles are lost by atresia before ovulation. It has become apparent that the apoptosis of granulosa cells induces follicular atresia. Forkhead box O3 (FOXO3), also called FKHRL1 (forkhead in rhabdomyosarcoma-like 1), is a proapoptotic molecule that belongs to the FOXO subfamily of forkhead transcription factors. Foxo3-deficient female mice were reported to be infertile because of abnormal ovarian follicular development, but the precise influences of FOXO3 on follicular atresia of mature ovary have not been determined. Therefore, we examined the expression and function of FOXO3 in porcine ovarian follicles and granulosa-derived cells. FOXO3 mRNA levels in granulosa cells of porcine ovaries increased during atresia, while FOXO3 protein was abundant in granulosa cells of early atretic follicles. By immunohistochemistry, the inner surface area of the granulosa layer in early atretic follicles was strongly stained with anti-FOXO3 antibody. The granulosa cells expressing FOXO3 coincided with apoptotic cells, indicating a role of FOXO3 as a proapoptotic factor in granulosa cells of porcine ovaries. In porcine (JC-410) and human (KGN) granulosa-derived cells, cell death was induced by transfection of FOXO3 expression vectors. Expression of the proapoptotic factors Fas ligand (FASLG) and BCL2-like 11 (BCL2L11) was upregulated by FOXO3 in KGN cells. In conclusion, FOXO3 is expressed in porcine ovarian follicles and induces apoptosis in granulosa cells, suggesting that it is a candidate for the initiator of follicular atresia. |
| | |
Authors:
|
Fuko Matsuda; Naoko Inoue; Akihisa Maeda; Yuan Cheng; Takafumi Sai; Hiroshi Gonda; Yasufumi Goto; Kazuhiro Sakamaki; Noboru Manabe |
Related Documents
:
|
3023146 - In vitro recovery of human chorionic gonadotropin-stimulated cyclic adenosine 3',5'-mon... 16726376 - Absence of specific follicle stimulating hormone receptors in porcine corpora lutea. 1370166 - Differential expression of aminopeptidase-n on human ovarian granulosa and theca cells. 2173616 - Regulation of steroidogenesis of infant and adult rhesus monkey granulosa cells in vitro. 3499766 - The role of folylpolyglutamate synthetase and gamma-glutamyl hydrolase in altering cell... 3742066 - Cell cycle analysis of estrogen stimulation and antiestrogen inhibition of growth of th... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-06 |
Journal Detail:
|
Title: The Journal of reproduction and development Volume: 57 ISSN: 1348-4400 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-03-22 Completed Date: 2011-08-04 Revised Date: 2011-12-13 |
Medline Journal Info:
|
Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: Japan |
Other Details:
|
Languages: eng Pagination: 151-8 Citation Subset: IM |
Affiliation:
|
Research Unit for Animal Life Sciences, Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Ibaraki, Japan. fukomat@yahoo.co.jp |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/AB433530; RefSeq/NM_001135959 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Apoptosis* Apoptosis Regulatory Proteins / metabolism Cell Line Fas Ligand Protein / metabolism Female Follicular Atresia / metabolism* Forkhead Transcription Factors / chemistry, genetics, metabolism, physiology* Gene Expression Regulation* Granulosa Cells / cytology, metabolism* Humans Membrane Proteins / metabolism Molecular Sequence Data Organ Specificity Ovarian Follicle / cytology, metabolism Protein Isoforms / genetics, metabolism Proto-Oncogene Proteins / metabolism RNA, Messenger / chemistry, metabolism Sequence Alignment Sequence Homology, Amino Acid Sexual Maturation Sus scrofa |
| Chemical | |
Reg. No./Substance:
|
0/Apoptosis Regulatory Proteins; 0/Bcl-2-like protein 11; 0/FOXO3 protein, human; 0/Fas Ligand Protein; 0/Forkhead Transcription Factors; 0/Membrane Proteins; 0/Protein Isoforms; 0/Proto-Oncogene Proteins; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: High-density lipoprotein cholesterol, obesity, and mammographic density in Korean women: the Healthy...
Next Document: Mitochondrial dysfunction influences apoptosis and autophagy in porcine parthenotes developing in vi...