| Expression of Fas ligand in arteries of hypercholesterolemic rabbits accelerates atherosclerotic lesion formation. | |
| | |
MedLine Citation:
|
PMID: 10669624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fas ligand (FasL) is expressed by cells of the arterial wall and is present in human atherosclerotic lesions. However, the role of FasL in modifying the initiation and progression of atherosclerosis is unclear. To investigate the role of arterial FasL expression in the development of atherosclerosis, we first established a model of primary lesion formation in rabbit carotid arteries. In this model, infusion of adenoviral vectors into surgically isolated, nondenuded arteries of hypercholesterolemic rabbits leads to the formation of human-like early atherosclerotic lesions. Expression of FasL in arterial endothelium in this model decreased T-cell infiltration and expression of vascular cell adhesion molecule-1 but did not affect expression of intercellular adhesion molecule-1. Intimal lesions grew more rapidly in FasL-transduced arteries than in arteries transduced with a control adenovirus that did not express a transgene. Total intimal macrophage accumulation was increased in FasL-transduced arteries; however, the proportion of lesion area occupied by macrophages was not elevated. The accelerated lesion growth was primarily due to the accumulation of intimal smooth muscle cells with a synthetic proliferative phenotype. There was no significant apoptosis in FasL-transduced or control arteries and no granulocytic infiltrates. Thus, the net result of elevated FasL expression is to accelerate atherosclerotic lesion growth by increasing lesion cellularity. Vascular expression of FasL may contribute to the progression of atherosclerosis. |
| | |
Authors:
|
D B Schneider; G Vassalli; S Wen; R M Driscoll; A B Sassani; M B DeYoung; R Linnemann; R Virmani; D A Dichek |
Related Documents
:
|
10073974 - Strong induction of members of the chitinase family of proteins in atherosclerosis: chi... 679694 - Angiometric assessment of coronary atherosclerosis in ischaemic heart disease in elderl... 2012724 - Atherosclerosis-related measures in digitized femoral arteriograms. 1768684 - Atherometric system. a suitable methodology to characterize atherosclerotic lesions in ... 22987234 - Compressive syndrome of internal jugular veins in multiple sclerosis: does it matter? 3556184 - Differential effects of prostaglandins on mesenteric and cerebral arteries isolated fro... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 20 ISSN: 1079-5642 ISO Abbreviation: Arterioscler. Thromb. Vasc. Biol. Publication Date: 2000 Feb |
Date Detail:
|
Created Date: 2000-03-03 Completed Date: 2000-03-03 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9505803 Medline TA: Arterioscler Thromb Vasc Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 298-308 Citation Subset: IM |
Affiliation:
|
Gladstone Institute of Cardiovascular Disease, University of California, San Francisco 94141-9100, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenoviridae
/
physiology Animals Apoptosis / physiology Arteries / metabolism* Arteriosclerosis / etiology*, metabolism, pathology Cell Division / drug effects Endothelium, Vascular / metabolism Fas Ligand Protein Gene Expression Hypercholesterolemia / complications*, metabolism* Macrophages / pathology Membrane Glycoproteins / genetics, metabolism*, pharmacology Muscle, Smooth, Vascular / cytology, drug effects, physiology Rabbits T-Lymphocytes / physiology Transgenes / genetics Vascular Cell Adhesion Molecule-1 / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
HL-59963/HL/NHLBI NIH HHS; HL-60504/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/FASLG protein, human; 0/Fas Ligand Protein; 0/Membrane Glycoproteins; 0/Vascular Cell Adhesion Molecule-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Macrophage scavenger receptor class A: A multifunctional receptor in atherosclerosis.
Next Document: Vascular endothelial cells and smooth muscle cells differ in expression of Fas and Fas ligand and in...