Document Detail


Expression of Ep-CAM in cervical squamous epithelia correlates with an increased proliferation and the disappearance of markers for terminal differentiation.
MedLine Citation:
PMID:  8774141     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ep-CAM, an epithelial adhesion molecule, is absent in normal squamous epithelia but can be detected in some squamous carcinomas. Using a panel of monoclonal antibodies to keratinocyte differentiation and proliferation markers, we investigated the association of EP-CAM expression with differentiation-related and/or neoplastic changes in cervical epithelium. Normal endocervical glandular epithelium (Both columnar and reserve cells) appeared strongly positive for EP-CAM, whereas ectocervical squamous epithelial cells did not express this molecule. Expression of Ep-CAM (in basal cells) was sometimes observed in morphologically normal ectocervical tissue but only in areas bordering cervical intraepithelial neoplasia (CIN) lesions. At the early stages of neoplasia the expression of Ep-CAM was regularly present in squamous epithelium, in general consistent with the areas of atypical, undifferentiated cells. Thus, in CIN grades I and II, the basal/suprabasal layers of the epithelia were positive, whereas in CIN grade III lesions, up to 100% of the cells over the whole thickness of the epithelium sometimes excluding the very upper layers, expressed Ep-CAM. A clear increase, not only in number of positive cells but also in levels of Ep-CAM expression (intensity) was observed during progression from CIN I to CIN III. Expression of Ep-CAM in ectocervical lesions did not coincide with a reappearance of the simple epithelium cytokeratins (CK8 and CK18). On the other hand, expression of Ep-CAM in atypical cells of CIN lesions correlated with the disappearance of CK13, which normally marks cells undergoing squamous differentiation. As was shown with Ki-67, a marker for proliferating cell populations, the areas of Ep-CAM expression were also the areas of enhanced proliferation. Cells expressing Ep-CAM did not express involucrin, a marker for cells committed to terminal differentiation. In the majority of both squamous and adenocarcinomas of the cervix a strong expression of Ep-CAM was observed, although some decrease in the expression (both the intensity and the number of positive cells), as compared with CIN III lesions, was observed in the areas of squamous differentiation. This study demonstrates that the expression of Ep-CAM in cervical squamous epithelium is associated with abnormal proliferation of cell populations that are not committed to terminal differentiation.
Authors:
S V Litvinov; W van Driel; C M van Rhijn; H A Bakker; H van Krieken; G J Fleuren; S O Warnaar
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of pathology     Volume:  148     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1997-01-08     Completed Date:  1997-01-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  865-75     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, Leiden University, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Neoplasm / metabolism*
Biological Markers
Cell Adhesion Molecules / metabolism*
Cell Differentiation
Cell Division
Cervical Intraepithelial Neoplasia / metabolism
Cervix Uteri / cytology*,  metabolism*,  pathology
Epithelium / metabolism
Female
Humans
Keratins / metabolism
Metaplasia
Tumor Markers, Biological
Uterine Cervical Dysplasia / metabolism
Uterine Cervical Neoplasms / metabolism
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Biological Markers; 0/Cell Adhesion Molecules; 0/Tumor Markers, Biological; 0/tumor-associated antigen GA733; 68238-35-7/Keratins
Comments/Corrections

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