| Expression of Endometrial Immune-related Genes Possibly Functioning During Early Pregnancy in the Mare. | |
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MedLine Citation:
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PMID: 23138119 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Despite enormous efforts, biochemical and molecular mechanisms associated with equine reproduction, particularly processes of pregnancy establishment, have not been well characterized. Previously, PCR-selected suppression subtraction hybridization analysis was executed to identify unique molecules functioning in the equine endometrium during periods of pregnancy establishment, and granzyme B (GZMB) cDNA was found in the pregnant endometrial cDNA library. Because GZMB is produced from natural killer (NK) cells, endometrial expression of GZMB and immune-related transcripts were characterized in this study. The level of GZMB mRNA is higher in the pregnant endometrium than in non-pregnant ones. This expression was also confirmed through Western blot and immunohistochemical analyses. IL-2 mRNA declined as pregnancy progressed, while IL-15, IFNG and TGFB1 transcripts increased on day 19 and/or 25. Analyses of IL-4 and IL-12 mRNAs demonstrated the increase in these transcripts as pregnancy progressed. Increase in CCR5 and CCR4 mRNAs indicated that both Th1 and Th2 cells coexisted in the day 25 pregnant endometrium. Taken together, the expression of immune-related transcripts suggests that immunological responses are present even before the trophectoderm actually attaches to the uterine epithelial cells. |
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Authors:
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Yurika Tachibana; Yasuko Nakano; Kentaro Nagaoka; Masato Kikuchi; Yasuo Nambo; Shingo Haneda; Motozumi Matsui; Yo-Ichi Miyake; Kazuhiko Imakawa |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-09 |
Journal Detail:
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Title: The Journal of reproduction and development Volume: - ISSN: 1348-4400 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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